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To properly incubate a TSA (Tryptic Soy Agar) plate, first ensure that the agar surface is inoculated with the sample of interest using sterile techniques. After inoculation, the plate should be inverted (agar side up) to prevent condensation from the lid dripping onto the agar surface. Incubate the plate at the appropriate temperature, typically 30-37°C, depending on the organism being cultured, and for the recommended duration, usually 24-48 hours. Finally, monitor for growth and contamination before proceeding with analysis.

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1mo ago

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