A centrifuge.
A watershed divide is a ridge of land that separates one watershed from another by directing the flow of surface water in different directions.
The line that separates one place from another is called a boundary. Boundaries can be physical, such as rivers or mountains, or they can be imaginary lines agreed upon by nations and jurisdictions.
The structure that separates one chamber of the heart from another is called Septum
The peritoneum (and the mesentary).
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The English Channel.
The line that seperates one air mass from another is called a boundary.
The membrane that separates one cell from another is called the plasma membrane or cell membrane. It is a phospholipid bilayer that regulates the passage of substances in and out of the cell.