How could you see zon layer in microscope?

Answer 1

To visualize the zonule layer (zonula occludens) in a microscope, you would typically use a transmission electron microscope (TEM) or a fluorescence microscope with specific staining techniques. TEM allows for high-resolution imaging of cellular junctions, revealing the tight junctions that characterize the zonule layer. In fluorescence microscopy, you can apply specific antibodies or fluorescent dyes that bind to proteins associated with tight junctions, enabling the visualization of the zonule layer in tissue sections. Proper sample preparation and staining are crucial for clear observation.