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Osprey were most susceptible because they were higher up the food chain.

*DDT runoff into streams *Minnows, crayfish, insects consumed some *Small fish ate those creatures, and so got an additive dose of all the DDT they were carrying *Larger fish ate the smaller ones and, again, the DDT load of each smaller fish added to that *Osprey consume large fish. THUS the osprey, as an end predator consumed the most DDT. Factor in that, the higher up the food chain you go, the fewer individuals there are.

The DDT made the eggshells fragile, so fewer osprey were hatched. So their numbers were reduced even more in comparison to those organisms that were more abundant to begin with.

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