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The best way to tell what gender a cat is, is by looking underneath the base of its tail. A male cat that has not been neutered will have two furry testicles right below the anus, and a penis right below the testicles. A neutered male cat will not have visible testicles, but there will be a noticeable space between its anus and penis.

Spayed and intact female cats alike will have a genital opening (vulva) which a tear-drop shape located right below the anus.

It is very difficult to accurately tell the sex of a kitten until it is at the very least two weeks old. In males, the testicles do not begin to develop until four to six weeks old.

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