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Generally by 5 weeks you will really notice slight differences in comb size, length of the legs, size of the feet and presence of saddle feathers.

Day one if you are VERY EXPERIENCED. Most hatcheries have people on staff (usually Japanese based on the need for sexing in a country with limited space) that sex day old chicks. They squeeze the chick to release fecal material then flip it over, open the vent and look for a small lump to determine male or female.

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