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It is an important technique for powdered drug ,especially when chemical & other method fail as accurate measure of quality. Lycopodium is composed of spores of lycopodium elavatum.I. Each spore is tetrahedral in shape, The base is rounded and the three

side wall

makes the three well marked covering ridge, which join one other at filled with fixed oil. The spore are exceptionally uniform in size(25µm) and the shape tetrahedral so that one can always know that a definite no. of spore present in particular weight of lycopodium. On an average 94000 spores per mg of powdered lycopodium are present. Using this figure one can calculate the weight of any number of spores under any condition under

the microscope.

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