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break the S-S bonds in a protein

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Q: What is the need for using edta in sds page?
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What is the function of glycine in sds page?

glycine molecular weight high so mobility also high so using in SDS PAGE


What is observed when run a sds-page using the pellet from serum collection?

SDS is a type of polyacrylamide gel in which bacteria can be grown. To see what can be observed, the collection and experiment should be done by the student.


Why is denaturing sds-page used for running sds-page electrophoresis of egg-white lysozyme and not non-denaturing page?

may be because of toomany disulfide linkages


Why p53 is run as 53 kda on sds page?

Due to many proline residues it migrates slower on sds page and appears heavier than it is.


What are the drawbacks of sds page?

The major drawback is that treatment with SDS denatures the protein, meaning you are not looking at it in its natural state.


What is Laemmli gels?

It is the gel of choice for SDS PAGE


What is SDS buffer?

SDS or sodiumdodecyl sulfate is a detergent used in protein separation. SDS buffer or SDS sample buffer consist of SDS, Tris, glycerol, bromo phenol blue, EDTA, and DTT or beta mercapto ethanol as a standard recipe. SDS is also added in stacking and separating gel preparation buffers that contain acrlamide.The main purpose is to keep the proteins denatured and provide the net negative charge to proteins as well as to run them according to it molecular weight


Who discovered SDS PAGE electrophoresis?

Laemmli U. K.


What test has replaced the radial immunodiffusion test?

SDS-PAGE method


What is role of glycine in SDS-PAGE?

Glycine increases the mobility of the gel.


What method could you use to further separate two bands from the same protein fraction after SDS-PAGE?

Electrophoresis is the method that could be used to further separate two bands from the same protein fraction after SDS-PAGE.


What is tens buffer?

Tens buffer is made of: Tris: For maintaining pH EDTA: acts as chelating agent SDs: Detergent for denaturation of protein NaOH: Strong base, destabilises charge