Serology for brucellosis

Answer 1

Definition

Serology for brucellosis is a blood test to look for antibodies against Brucella, the bacteria that causes the disease brucellosis.

Alternative Names

Brucella serology; Brucella antibody test or titer

How the test is performed

Blood is typically drawn from a vein, usually from the inside of the elbow or the back of the hand. The site is cleaned with germ-killing medicine (antiseptic). The health care provider wraps an elastic band around the upper arm to apply pressure to the area and make the vein swell with blood.

Next, the health care provider gently inserts a needle into the vein. The blood collects into an airtight vial or tube attached to the needle. The elastic band is removed from your arm. Once the blood has been collected, the needle is removed, and the puncture site is covered to stop any bleeding.

In infants or young children, a sharp tool called a lancet may be used to puncture the skin and make it bleed. The blood collects into a small glass tube called a pipette, or onto a slide or test strip. A bandage may be placed over the area if there is any bleeding.

The blood is then tested in a laboratory to look for antibodies. For Brucella, the serum agglutination test (SAT) is the simplest and most widely used testing method.

How to prepare for the test

There is no special preparation.

How the test will feel

When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.

Why the test is performed

This test may be performed when the doctor suspects brucellosis.

Normal Values

A normal result shows no antibodies to Brucella. However, during the first few days to weeks of exposure to an antigen, there may be very little antibody production. Therefore, a serology test may not detect early stage disease.

As brucellosis progresses, more antibodies will be present. If the health care provider suspects brucellosis, you may need to have the test repeated 10 days to 2 weeks after the first test to watch for this rise.

Infection with other bacteria, such as Yersinia, Francisella, and Vibrio, and immunizations can make the test falsely positive.

Normal value ranges may vary slightly among different laboratories. Talk to your doctor about the meaning of your specific test results.

What abnormal results mean

If the test detects antibodies, you have likely been exposed to the Brucella bacteria (possible brucellosis).

What the risks are

Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.

Other risks associated with having blood drawn are slight but may include:

• Excessive bleeding
• Fainting or feeling light-headed
• Hematoma (blood accumulating under the skin)
• Infection (a slight risk any time the skin is broken)

Special considerations

A serology test can determine if you have ever been exposed to a particular antigen, but this does not necessarily mean that you are currently infected. Increasing antibody levels over a 2-week period are more likely to indicate a current infection.

References

Salata RA. Brucellosis. In: Goldman L, Ausiello D, eds. Cecil Medicine. 23rd ed. Philadelphia, PA: Saunders Elsevier; 2007: chap 331.

Answer 2

Definition

Serology for brucellosis is a blood test to look for antibodies against Brucella, the bacteria that causes the disease brucellosis.

Brucellosis is an infection that occurs from coming into contact with animals carrying Brucella bacteria.

Alternative Names

Brucella serology; Brucella antibody test or titer

How the test is performed

A blood sample is needed. For information on how this is done, see: Venipuncture

The blood is then tested in a laboratory to look for antibodies. For Brucella, the serum agglutination test (SAT) is the simplest and most widely used testing method.

How to prepare for the test

There is no special preparation.

How the test will feel

When the needle is inserted to draw blood, some people feel moderate pain, while others feel only a prick or stinging sensation. Afterward, there may be some throbbing.

Why the test is performed

Your doctor may order this test if you have signs or symptoms of brucellosis. People working in jobs where they often come in contact with animals or meat, such as slaughterhouse workers, farmers, and veterinarians, are most likely to get this disease.

Normal Values

A normal (negative) result usually means you have not come in contact with the bacteria that causes brucellosis. However, this test may not detect the disease at an early stage. Your doctor may have you come back for another test in 10 days to 3 weeks.

Infection with other bacteria, such as Yersinia, Francisella, and Vibrio, and certain immunizations can cause false-positive results.

Normal value ranges may vary slightly among different laboratories. Some labs use different measurements or test different samples. Talk to your doctor about the meaning of your specific test results.

What abnormal results mean

An abnormal (positive) results usually means you have come in contact with the bacteria that causes brucellosis.

However, this does not mean that you have an active infection. Your doctor will repeat the test after a few weeks to see if the test result increases. This is more likely to be a sign of a current infection.

What the risks are

Veins and arteries vary in size from one patient to another and from one side of the body to the other. Obtaining a blood sample from some people may be more difficult than from others.

Other risks associated with having blood drawn are slight but may include:

• Excessive bleeding
• Fainting or feeling light-headed
• Hematoma (blood accumulating under the skin)
• Infection (a slight risk any time the skin is broken)

References

Salata RA. Brucellosis. In: Goldman L, Schafer AI, eds. Cecil Medicine. 24th ed. Philadelphia, PA: Saunders Elsevier; 2011:chap 318.

Reviewed By

Review Date: 12/06/2011

David C. Dugdale, III, MD, Professor of Medicine, Division of General Medicine, Department of Medicine, University of Washington School of Medicine; Jatin M. Vyas, MD, PhD, Assistant Professor in Medicine, Harvard Medical School, Assistant in Medicine, Division of Infectious Disease, Department of Medicine, Massachusetts General Hospital. Also reviewed by David Zieve, MD, MHA, Medical Director, A.D.A.M., Inc.