To identify the pure culture the best method is to perform sub culturing and it can be done either by on another agar plate by streak plate method or by pour plate method
The next is to perform staining which give the difference in morphology if the culture is not pure. But we should perform both the test and then should corelate them because none of them is individually 100% full proof
yes, just the mix culture need to be separated firts
In the slant culture, the bacterial growth is even to spreading to irregular. On the other hand, in broth culture, the growth ranges from sedimentation at the bottom, to turbid growth to pellicle growth.
a slant broth is a type of inoculating agar to gather bacterial data from.
So that you can be sure that the results are due only to one specific species.
You have to make subculture from this slant and after incubation you can observe how many types of microorganisms are present in the nutrient agar slant. If you have one colony shape so you have a pure nutrient agar slant but if you have more than one type of colonies so the nutrient agar slant is contaminated.
Anarobes can be inoculated in an agar slant using an inoculating needle. The needle is able to put the anarobes deep inside the agar. The anarobes can then grow in the anarobic environment provided. The agar slant should then be refrigerated after suitable growth is achieved.
In the slant culture, the bacterial growth is even to spreading to irregular. On the other hand, in broth culture, the growth ranges from sedimentation at the bottom, to turbid growth to pellicle growth.
a slant broth is a type of inoculating agar to gather bacterial data from.
Pure broth culture is a liquid media, used to propagate large numbers of microorganisms. Where as Slant cultures are semi solid media containing a solidifying agent (usually agar). Can be used in determining bacterial motility and in promoting anaerobic growth.
So that you can be sure that the results are due only to one specific species.
in the broth they will be packed close together and more spread out in the slant
generally slant is prepared in test tube because test tube provide more surface area for microbial growth and the use of test tube in slanting is that test tube is best equipment to store media for long time.
Funky slant-eyed Asian niggurs in Czech Republic?
You have to make subculture from this slant and after incubation you can observe how many types of microorganisms are present in the nutrient agar slant. If you have one colony shape so you have a pure nutrient agar slant but if you have more than one type of colonies so the nutrient agar slant is contaminated.
Anarobes can be inoculated in an agar slant using an inoculating needle. The needle is able to put the anarobes deep inside the agar. The anarobes can then grow in the anarobic environment provided. The agar slant should then be refrigerated after suitable growth is achieved.
stroke culture is made in tubes containing agar slopes(slant) and is employed for providing a pure growth of bacterium for slide agglutination n other diagnostic tests. lawn cultures are prepared by flooding the surface of the plate with a liquid culture or suspension of bacterium n incubating the plate and is employed when a large amount of growth is required on solid media eg.preparation of bacterial antigens and vaccines.
If you are talking about media, then the blue is used as an indicatior for when a certain culture of microorganisms grow.
slant hemming