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What is one way to determine whether a bacterial culture has received a recombinant plasmid?
Wiki User
∙ 13y ago
The plasmid have a "reporter gene" inside it, generally resistance to specific antibiotic. the plasmid is transformed into bacteria that don't have resistance to that specific antibiotic drug, and than the cultured on a petri-dish that contain the antibiotic drug. Only bacteria that had receive the plasmid will have resistance and grow, all the other will die.
Wiki User
∙ 16y ago
Wiki User
∙ 13y agoPurify it. Using columns. This works when you are identifying a plasmid that has been recombined with genes that will make it apparent (ie P-GLO), otherwise you will need some experience and a good lab.
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Determine the population of bacteria based on the bacterial colonies?
Assume each colony started as a single bacteria in the original culture. Count the colonies you have and multiply up according to how diluted you made the culture and how much of the original culture you used.
What is the role of Cloning Host in recombinant DNA technology?
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.
What is a bacterial culture containing a single species of organisms referred to as?
A bacterial culture containing a single species of organism is referred to as pure or axenic.
What is the disadvantage of using more than 3 day old bacterial culture?
is it accurate to use a 3 day old bacterial culture
What type of culture medium would increase the size of a bacterial capsule?
it depends on the bacterial capsule, some grows on glucose, some grow on carbondioxide and low iron(LIM culture).
Determine the population of bacteria based on the bacterial colonies?
Assume each colony started as a single bacteria in the original culture. Count the colonies you have and multiply up according to how diluted you made the culture and how much of the original culture you used.
What are the types of sputum cultures?
Bacterial culture. Fungal culture. Viral culture
What is the role of Cloning Host in recombinant DNA technology?
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.
What is the disadvantage of using more than 3-day old bacterial culture?
is it accurate to use a 3 day old bacterial culture
What is a bacterial culture containing a single species of organisms referred to as?
A bacterial culture containing a single species of organism is referred to as pure or axenic.
What is the disadvantage of using more than 3 day old bacterial culture?
is it accurate to use a 3 day old bacterial culture
How does a recombinant vector work?
Recombinant vectors or Viral vectors are tools commonly used by molecular biologists to deliver genetic material into cells. This process can be performed inside a living organism (in vivo) or in cell culture (in vitro).
Is a yogurt a virus or a bacteria?
It's a bacterial culture.
Is OLD cheese good for you?
Yes it is. The bacterial culture is the same.
Can you innoculate bacterial culture in a plastic centrifuge tube?
yes.
Blood test that check for bacterial growth?
A blood culture
What type of culture medium would increase the size of a bacterial capsule?
it depends on the bacterial capsule, some grows on glucose, some grow on carbondioxide and low iron(LIM culture).
