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How do you find genes?

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11y ago

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There are several ways of finding genes, including:

- labeling and detecting DNA binding proteins that you think may bind to your gene of interest (DNA combing)

- taking the cDNA of a protein coding gene and tracing it back into the genome

- making a labeled complimentary probe of the gene, denaturing the genome, and hoping that your probe finds its complimentary match

- sequencing and trying to locate regions that look like a predicted sequence

- transforming a population of cells with a vector (that contains a selectable marker) of the organism of interest's genome, and selecting for that gene using that marker or different properties of the gene (especially if the species of cells you've chosen can transcribe/translate inserted genes)

There are probably numerous more general methods, but that's just a few :)

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