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== == When using a light microscope you encounter diffraction. (Visible light behaves like a wave, with a wavelength of about 300 to 900 nanometres). We say that light diffracts when its wavelike behaviour makes it bend around obstacles, or spread out. If the obstacle is much larger than the wavelength of the incoming wave, the spreading-out will be smaller. If the gap is smaller than the wavelength, then the spreading-out will be very large. In a light microscope, the light waves will spread out whenever they pass through a lens, or any sort of obstruction. The primary lens in most microscopes is of much bigger than 300-900 nanometres, so the angle by which light waves diverge is really quite small. However, it is not zero the light waves do spread out a little bit, and the result is that the visual field is always a bit blurry. It is impossible for an ordinary light microscope to avoid this problem, so they can never see structures smaller than about 500 nm.

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16y ago
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9y ago

A compound microscope is limited to a magnification of around 2000X. Beyond this limit, the image under the microscope will be unrecognizable.

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13y ago

wavelength of light used and the diffraction effect.

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12y ago

The resolving power of the microscope.

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Q: What limits the resolving power of a light microscope?
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Related questions

What is the resolving power of a scanning electron microscope?

the resolving power of light microscope is 0.2 micrometr


Explain the relationship between the resolving power of the microscope and the wavelength of the light being used?

Resolving power of microscope is inversely related to the wavelength of the light used. So shorter the wavelength, greater the resolving power.


What is the resolving power of a light microscope?

Around 0.2micrometers or 200 nm


What limits the resolving power of an electron microscope?

Since there might be problems with the specimen preparation.


The magnification of a light microscope is limited to 1000X by the resolving power of the instrument.?

True!


Which has the least resolving power?

A transmission electron microscope.


What is the resolving power of a compound microscope?

.2 um


What happens to contrast and resolving power when the aperture of the condenser of a compound microscope is decreased?

That will depend whether the microscope is designed to cope with the new wavelength as well as it did with the old. For example, ordinary visible-light microscopes are useless for ultraviolet. The absolute limit to resolving power with perfect optics is about quarter of a wavelength but real microscopes fall short of this.


What does the power of a microscope depend on?

Depends which type of microscope we are talking about. The common compund light microscope has a resolving power of 0.2 micrometer or 0.0002 millimeter. In comparison the human eye's resolving power is 0.1 millimeter. Resolving power is the minimum distance between two objects or particles such that the objects are distinguishable. So for example in the case of human eyes with resolving power of 0.1 millimeter, if you bring two objects any closer to each other than 0.1 mm, our eye cannot tell if they are two separate objects or not. Last but not least, the lower the resolving power, the higher the resolution. So because a compound microscope has a lower resolving power than human eye, it has a higher resolution.


What is the resolving power of an electron microscope?

Resolving power is the ability of an imaging device to separate points of an object that are located at a small angular distance


The ability of a microscope to reveal fine detail is known as?

Resolving Power


A microscope that can magnify up to 500 000 times is the?

A electron microscope uses a beam of electrons instead of light to magnify objects up to 500,000 times actual size. A electron microscope has much higher resolving power than light microscopes.