0
Isotopes are variants of a particular chemical element which differ in neutron number, although all isotopes of a given element have the same number of protons in each atom. Isotope Variation Analysis (detection of adulteration in food products or the geographic origins of products using isoscapes) and Isotopic substitution (to determine the mechanism of a chemical reaction via the kinetic isotope effect) are the most frequently used application for isotopes. Another common application for isotope is isotopic labeling, the use of unusual isotopes as tracers or markers in chemical reactions. Normally, atoms of a given element are indistinguishable from each other. However, by using isotopes of different masses, even different nonradioactive stable isotopes can be distinguished by mass spectrometry or infrared spectroscopy. For example, in 'stable isotope labeling with amino acids in cell culture (SILAC)' stable isotopes are used to quantify proteins. If radioactive isotopes are used, they can be detected by the radiation they emit (this is called radioisotopic labeling).
Wiki User
∙ 6y ago
Add your answer:
Earn +20 pts
What is meant by hot and cold radio-isotope labeling with reference to DNA sequencing?
What they mean by "hot" is when the substance is "radioactive" and as such it could be detected by an X-ray film. I am not to sure with the "cold" method.
When a radioactive tracer is used why is it best to use a radioactive isotope that decays into a stable isotope?
The principle behind the use of radioactive tracers is that an atom in a chemical compound is replaced by another atom, of the same chemical element. The substituting atom, however, is a radioactive isotope. This process is often called radioactive labeling.
What is C-14 used for?
it is used as an isotope and it can be used for good or super villain purposes.
What is the process in which one isotope changes to another isotope?
Radioactive decay is the process in which one isotope is changed into another isotope.
What is a Cosmogenic isotope?
a cosmogenic isotope is an isotope that is created in space by cosmic rays
What is the radio labeling technique?
Radio labeling is incorporation of a radioactive isotope within a molecule , so the compound can be detected using radioactivity detection techniques.
What stable isotopes are used in labeling peptides?
Stable isotopes used in labeling peptides may include 2H, 15N & 13C, respectively, or a combination of 15N & 13C.Source: a passage named Stable Isotope Labeled Peptideson the website of Creative Peptides.
What has the author Robert R Wolfe written?
Robert R. Wolfe has written: 'Isotope tracers in metabolic research' -- subject(s): Metabolism, Methodology, Radioactive tracers in biochemistry, Research 'Radioactive and stable isotope tracers in biomedicine' -- subject(s): Isotope Labeling, Mass Spectrum Analysis, Metabolism, Methodology, Methods, Radioactive tracers in biochemistry, Research
What has the author Erwin Regoeczi written?
Erwin Regoeczi has written: 'Iodine-Labeled Plasma Proteins' -- subject(s): Analysis, Blood proteins, Iodine, Isotope Labeling, Isotopes, Radioiodination
What is meant by hot and cold radio-isotope labeling with reference to DNA sequencing?
What they mean by "hot" is when the substance is "radioactive" and as such it could be detected by an X-ray film. I am not to sure with the "cold" method.
When a radioactive tracer is used why is it best to use a radioactive isotope that decays into a stable isotope?
The principle behind the use of radioactive tracers is that an atom in a chemical compound is replaced by another atom, of the same chemical element. The substituting atom, however, is a radioactive isotope. This process is often called radioactive labeling.
What is school labeling?
If you mean as in School labeling (labeling people) Its when someone calls you like an emo or a geek etc ...
What affects half-life?
The half-life of a radioactive isotope is not affected by environmental conditions, such as temperature and pressure. For all practical purposes, it can be considered as fixed.
A sentence using and labeling verb?
My teacher was labeling the classroom;
What is C-14 used for?
it is used as an isotope and it can be used for good or super villain purposes.
Is civil partnership wrong?
No. Civil partnership is perfectly legal in the places where it is permitted. People generally have good intentions and noble purposes when they enter into civil partnerships. There is no rational basis for labeling civil partnership as "wrong."
What is the process in which one isotope changes to another isotope?
Radioactive decay is the process in which one isotope is changed into another isotope.
