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Watch out for the fire as it might burn the equipment
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∙ 12y ago
Wiki User
∙ 11y agoDont touch the burner.
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Aseptic technique correct sequence of events in transferring bacteria from a culture tube into a new media tube?
1) Light a Bunsen burner and hold a transfer loop (*or needle) over the flame until it becomes red hot (hottest place is just above the first blue flame). a. *loops are used for transfers to broth media, needles are for transfer to agar media 2) Hold both test tubes in one hand at an angle (be sure not to spill, use your non-dominate hand, and have the mouth sections of the tubes set up above your hand). In the other hand you should be holding the sterile transfer tool with your thumb and forefinger, take the tube caps off with your other three fingers. (be sure note which cap goes with which tube and not to mix them up) 3) Quickly pass the top sections of the tubes through the Bunsen flame. 4) Dip the needle or loop into the broth culture and stir around. a. When using a loop be sure to get a drop in the middle. 5) Take out the tool without touching the sides of the tube and transfer the bacteria into the other tube media. (loops for broth media should be stirred, needles for agar media should be stabbed straight down almost to the butt of the tube and takes back straight out) 6) Take the inoculation tool out (continue holding) and sterilize the tube tops again (having been kept at a slant the entire time) and replace the correct caps. 7) Sterilize the inoculation tool in the Bunsen flame again before putting it down anywhere.
What are 4 facts about the Bunsen Burner?
The Bunsen burner is such a familiar fixture of chemistry labs that its reputation reaches students even before they enter the classroom. As an icon of science, it permeates popular culture. But where did the Bunsen burner come from? Who invented it? You might hope to chuckle at the absurdly obvious: "why, Bunsen, of course!" But a brief foray into history may be warranted before placing too significant a wager on the "obvious."Robert Bunsen, whose name we associate with the burner, was a 19th-century German chemist of some renown. He worked on explosive organic arsenic compounds--leading to the loss of one eye--and, later, on gases from volcanoes, geysers and blast furnaces. With Kirchoff he contributed to our understanding of the meaning of spectra lines. (He also gained note for not bathing--one woman of polite society remarked that Bunsen was so charming that she would like to kiss him, but she would have to wash him first.) Bunsen invented many bits of laboratory apparatus: the spectroscope, the carbon-pole battery, an ice calorimeter and vapor calorimeter, the thermopile, and the filter pump--but not, as one might imagine, the gas burner that bears his name. Rather, the "Bunsen" burner was developed by Bunsen's laboratory assistant, Peter Desdega. Desdega himself likely borrowed from earlier designs by Aimé Argand and Michael Faraday. So why does Bunsen get the implicit credit? --And why do we know so little about Desdega that we cannot add much to his story?"Bunsen's" burner illustrates an important dimension of science frequently omitted in teaching about science: professional credit. Eponymous laws and labels--whose names reflect their discoverers--appear throughout science: Snell's law of refraction, Gay-Lussac's law of gases, the Hardy-Weinberg model of population genetics, the volt (named for Alexander Volta), etc. The naming of laws for their discoverers seems appropriate for honoring the scientists--and the human names are handy for reminding students that science is done by real persons. But in this system, one person and only one person gets all the credit. Focusing on great individuals can hide the collective nature of science, especially the role of technicians such as Desdega. How do we distribute the credit where appropriate?The great Isaac Newton is frequently quoted for expressing the humbling effect of the collective effort in science: "If I have seen further," he once professed, "it is by standing on the shoulders of giants." Newton's claim, we now know, betrayed a false modesty. Newton's bitter priority dispute with Leibniz over the invention of the calculus, in particular, bears witness to his ambition and obsession with prestige--and his political maneuvers in trying to achieve it. In that case, at least, Newton tried to further his own stature "by standing on the claims of competitors." In similar ways, perhaps, the contributions of technical workers often get buried when we allow theoretical discoveries of the work of their masters to overshadow them. Bunsen's burner--or perhaps the Desdega burner--is a notable example.The story of the Petri dish is an interesting exception--while at the same time underscoring the general pattern of invisible technicians. Julius Richard Petri (1852-1921) worked for the master of "germ theory" in Germany in the late 1800s, Robert Koch (1843-1910; pronounced as a gutteral "coke"). Initially, bacteria were cultured in liquid broth--a practice captured in our famous images of experiments on spontaneous generation. But Koch saw the advantage of growing bacteria on a solid medium instead. By spreading out mixtures of microorganisms on a solid surface, he could separate individual types in isolated colonies. With pure colonies, he could investigate the effects of each bacterium. The method allowed Koch to identify the specific organisms that cause tuberculosis, cholera, diptheria, among many other diseases--and then to develop vaccines.
What are two things the students should look at before evaluating which container is better for the environment?
They should examine things like: Can it be recycled? How much CO2 is produced to make or dispose of? How long do these things take to decompose? Is it biodegradable? Does the container leech toxic materials into the water or soil? Students who are using Ask.com to search for answers in their APEX coursework should note that they need to paraphrase their answers so their teachers (who are not stupid) do not give them back revisions for plagiarism ;).
In order for a effective periodic review you should?
So I taking the apex test and the answer for me was c: make note cards
How do you use nota bene in a sentence?
The term "nota bene" translates to English as "note well." An example of a sentence using this phrase would be: We will be in the Adirondacks this weekend; nota bene it gets very chilly at night in the mountains.
What to note of when using Bunsen burner?
dont burn things
How do you boil water using a Bunsen burner?
you put your water in the bursen burner then put in on heat the heat should be moderate to avoid vigorous boiling.Alway take note that you do not make the mouth of the bursen burner to face you put it the opposite side and do not lok in side the bursen burner.
What are the points to note in using the Bunsen burner?
- Never leave the gas on when the flame is out- never put anything you don't intend to heat- ensure the tube is arranged properly as if it is turning here and there, the gas might just be stucked somewhere and there will not be any fire burning- never touch the collar(the neck) as it is very hot and when it strikes back at the gashole, off the gas immediately.
Aseptic technique correct sequence of events in transferring bacteria from a culture tube into a new media tube?
1) Light a Bunsen burner and hold a transfer loop (*or needle) over the flame until it becomes red hot (hottest place is just above the first blue flame). a. *loops are used for transfers to broth media, needles are for transfer to agar media 2) Hold both test tubes in one hand at an angle (be sure not to spill, use your non-dominate hand, and have the mouth sections of the tubes set up above your hand). In the other hand you should be holding the sterile transfer tool with your thumb and forefinger, take the tube caps off with your other three fingers. (be sure note which cap goes with which tube and not to mix them up) 3) Quickly pass the top sections of the tubes through the Bunsen flame. 4) Dip the needle or loop into the broth culture and stir around. a. When using a loop be sure to get a drop in the middle. 5) Take out the tool without touching the sides of the tube and transfer the bacteria into the other tube media. (loops for broth media should be stirred, needles for agar media should be stabbed straight down almost to the butt of the tube and takes back straight out) 6) Take the inoculation tool out (continue holding) and sterilize the tube tops again (having been kept at a slant the entire time) and replace the correct caps. 7) Sterilize the inoculation tool in the Bunsen flame again before putting it down anywhere.
What are 4 facts about the Bunsen Burner?
The Bunsen burner is such a familiar fixture of chemistry labs that its reputation reaches students even before they enter the classroom. As an icon of science, it permeates popular culture. But where did the Bunsen burner come from? Who invented it? You might hope to chuckle at the absurdly obvious: "why, Bunsen, of course!" But a brief foray into history may be warranted before placing too significant a wager on the "obvious."Robert Bunsen, whose name we associate with the burner, was a 19th-century German chemist of some renown. He worked on explosive organic arsenic compounds--leading to the loss of one eye--and, later, on gases from volcanoes, geysers and blast furnaces. With Kirchoff he contributed to our understanding of the meaning of spectra lines. (He also gained note for not bathing--one woman of polite society remarked that Bunsen was so charming that she would like to kiss him, but she would have to wash him first.) Bunsen invented many bits of laboratory apparatus: the spectroscope, the carbon-pole battery, an ice calorimeter and vapor calorimeter, the thermopile, and the filter pump--but not, as one might imagine, the gas burner that bears his name. Rather, the "Bunsen" burner was developed by Bunsen's laboratory assistant, Peter Desdega. Desdega himself likely borrowed from earlier designs by Aimé Argand and Michael Faraday. So why does Bunsen get the implicit credit? --And why do we know so little about Desdega that we cannot add much to his story?"Bunsen's" burner illustrates an important dimension of science frequently omitted in teaching about science: professional credit. Eponymous laws and labels--whose names reflect their discoverers--appear throughout science: Snell's law of refraction, Gay-Lussac's law of gases, the Hardy-Weinberg model of population genetics, the volt (named for Alexander Volta), etc. The naming of laws for their discoverers seems appropriate for honoring the scientists--and the human names are handy for reminding students that science is done by real persons. But in this system, one person and only one person gets all the credit. Focusing on great individuals can hide the collective nature of science, especially the role of technicians such as Desdega. How do we distribute the credit where appropriate?The great Isaac Newton is frequently quoted for expressing the humbling effect of the collective effort in science: "If I have seen further," he once professed, "it is by standing on the shoulders of giants." Newton's claim, we now know, betrayed a false modesty. Newton's bitter priority dispute with Leibniz over the invention of the calculus, in particular, bears witness to his ambition and obsession with prestige--and his political maneuvers in trying to achieve it. In that case, at least, Newton tried to further his own stature "by standing on the claims of competitors." In similar ways, perhaps, the contributions of technical workers often get buried when we allow theoretical discoveries of the work of their masters to overshadow them. Bunsen's burner--or perhaps the Desdega burner--is a notable example.The story of the Petri dish is an interesting exception--while at the same time underscoring the general pattern of invisible technicians. Julius Richard Petri (1852-1921) worked for the master of "germ theory" in Germany in the late 1800s, Robert Koch (1843-1910; pronounced as a gutteral "coke"). Initially, bacteria were cultured in liquid broth--a practice captured in our famous images of experiments on spontaneous generation. But Koch saw the advantage of growing bacteria on a solid medium instead. By spreading out mixtures of microorganisms on a solid surface, he could separate individual types in isolated colonies. With pure colonies, he could investigate the effects of each bacterium. The method allowed Koch to identify the specific organisms that cause tuberculosis, cholera, diptheria, among many other diseases--and then to develop vaccines.
When would you use an open and closed flame?
The closed flame (orange) is only ever used when turning on the bunsen burner or when you are leaving it for any amount of time to insure the safety of your class mates. The open flame (blue) is used at all other times. Please note it can be hard to see the blue flame so it is important that you stay aware and safe as it is often as hot as 700oC.
When using cornell note taking style was is the last thing you should do?
The last step in the system is to review what you've learned.
What should you record on a note card if you're using the index card organizational system?
If you are using the index notecard organizational system to help write a report you should include the source information. You should also include the information that you want to remember.
What should you record on a note card if you're using the index-card organizational system?
If you are using the index notecard organizational system to help write a report you should include the source information. You should also include the information that you want to remember.
Major chords are formed by using notes of a major scale?
Yes, using the root note (tonic note) of the scale and its 3rd and 5th note of the scale.
Should the note you buzz be the note you hear?
yes
What should i do if I get a love note?
Attempt to find out who the note is from and propose.
