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EMB plate testing is used to look for bacteria with lactose fermentation. + test results show dark purple, blackish or metalic green bacteria colony. On EMB plate, following results showed with 4 different bacteria. * E. aerogenes: Purple colony (+) * E. coli: Metalic green colony (+) * S. aureus: No growth (-) * S. facealis: No growth (-) With the result, we can conclude that lactose fermentation occured by E. aerogeneus and E. coli.
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∙ 14y ago
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∙ 14y agoEMB is both selective and differential. It is selective for gram negative bacteria and differential for lactose fermentation
Intelligence is like a river the deeper it gets the less noise it makes
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∙ 12y agoamylase (enzyme created by certain bacteria that can break down starch). A clearing around growth indicates amylase is present.
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∙ 11y agogram positive
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∙ 13y agovirus
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Why must use starch casein nitrate agar and what is the starch casein agar properties?
Because it is a selective medium
Is E. coli positive or negative for starch agar test?
E.coli does not digest the starch on a starch agar plate, therefore it does not produce amylase making it negative.
Why do bacteria grow heavily on starch agar but don't produce alpha amylase?
The ingredients in starch agar consist of carbohydrate sources in addition to starch. For example, starch could be added to trypticase soy agar (TSA) to test for hydrolysis. Remember, microorganisms are highly versatile and just because they cannot utilize certain nutrients does not mean that they cannot grow in the presence of that substance (in this case starch).
What would be found in the clear area that would not be found in the blue area of a starch agar plate after the additon of iodine?
Another organism on the starch agar plate breaks down the starch into smaller sugars, and the starch intolerant organism in turn competes for the smaller sugars. As a result, you will see colonies of the starch user pop up first, and then smaller satellite colonies of the dependant organism will form around them.
Why should a positive result not be read before the incubation time is completed in a Starch agar test?
Okay I think I know how to answer this one; you want to give wait until the incubation time is completed for a starch agar test because the cells need to mature in order to produce the enzyme that breaks starch down into a usable form (since starch cannot enter the membrane without being simplified) I hope this helps
Why must use starch casein nitrate agar and what is the starch casein agar properties?
Because it is a selective medium
Is E. coli positive or negative for starch agar test?
E.coli does not digest the starch on a starch agar plate, therefore it does not produce amylase making it negative.
What are the contents of starch agar?
:D Beef extract (3.0g) Soluble Starch (10.0) Agar (12.0) Distilled water (1,000.0 ml)
When is a starch agar plate used?
Agar is used to culture bacteria for scientific research.
Why do bacteria grow heavily on starch agar but don't produce alpha amylase?
The ingredients in starch agar consist of carbohydrate sources in addition to starch. For example, starch could be added to trypticase soy agar (TSA) to test for hydrolysis. Remember, microorganisms are highly versatile and just because they cannot utilize certain nutrients does not mean that they cannot grow in the presence of that substance (in this case starch).
Why do you test for starch?
You can test for starch in food by dropping iodine on food on an agar plate, if it turns black, it contains starch.
How can organisms that dont use starch grow on a starch agar plate?
Organisms that do not use starch grows on a starch agar plate by using other organisms. The other organisms break down the starch into sugar and the starch intolerant organisms can complete those simple sugars.
What regent would you add to starch agar plate to detect starch hydrolsis?
To detect starch hydrolysis on a starch agar plate, you can add iodine solution. Iodine reacts with starch to form a blue-black color, and if starch is hydrolyzed by the organism, the clear zone around the bacterial growth will indicate the presence of amylase, an enzyme that breaks down starch.
What would be found in the clear area the would not be found in the blue area of a starch agar plate after the addition of iodine?
Another organism on the starch agar plate breaks down the starch into smaller sugars, and the starch intolerant organism in turn competes for the smaller sugars. As a result, you will see colonies of the starch user pop up first, and then smaller satellite colonies of the dependant organism will form around them.
What would be found in the clear area that would not be found in the blue area of a starch agar plate after the additon of iodine?
Another organism on the starch agar plate breaks down the starch into smaller sugars, and the starch intolerant organism in turn competes for the smaller sugars. As a result, you will see colonies of the starch user pop up first, and then smaller satellite colonies of the dependant organism will form around them.
Why should a positive result not be read before the incubation time is completed in a Starch agar test?
Okay I think I know how to answer this one; you want to give wait until the incubation time is completed for a starch agar test because the cells need to mature in order to produce the enzyme that breaks starch down into a usable form (since starch cannot enter the membrane without being simplified) I hope this helps
What is composition of starch agar plate?
The composition of staph 110 agar is: Yeast extract - 2.5 g/L Tryptone - 10.0 g/L Lactose - 2.0 g/L Mannitol - 10.0 g/L Sodium Chloride - 75.0 g/L Dipotassium hydrogen phosphate - 5.0 g/L Gelatin - 30.0 g/L Agar - 15.0 g/L
