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In paper chromatography n-butanol:acetic acid:water mixture in the ratio 4:1:1 is used as a solvent whereas in case of thin layer chromatography(TLC) petroleum ether and acetone in the ratio 9:1 is used as a solvent.
Also, in some cases, DCM(dichloromethane is used as a solvent.
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∙ 10y ago
Wiki User
∙ 13y agoAs such there is no hard and fast rule which solution to use. It depends on what component of the material are we interested in as carries the components of the mixture with it and different components travel at different rates.
Wiki User
∙ 12y agoi dont know what your talking about dude!
Wiki User
∙ 11y agowhatmann filter paper
Wiki User
∙ 11y agoBlack ink
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Why stop paper chromatography from reaching the top of the paper?
The end of the paper nearest the spot is allowed to be in contact with a suitable solvent, WITHOUT immersing the spot itself. If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself.
What are the relations with pigment and chromatography?
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
Is methanol a good solvent to use in paper chromatography?
Because methanol is a very polar solvent
What will happen if there is an overrun of solvent in paper chromatography?
The Rf value cannot be determined since the distance of the solvent may overlay in the paper.
What is meant by tailing a spot in Chromatography?
Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.
Why stop paper chromatography from reaching the top of the paper?
The end of the paper nearest the spot is allowed to be in contact with a suitable solvent, WITHOUT immersing the spot itself. If the spots were immersed they would dissolve in the solvent and be pulled down into the solvent itself.
Why solvent is used in Paper chromatography?
In chromatography you need one stationery phase (paper) and one mobile phase (here solvent, moving by capillary forces)
What are the relations with pigment and chromatography?
the more soluble the plant pigment is with the chromatography solvent, the farther it will travel up the chromatography paper
Is methanol a good solvent to use in paper chromatography?
Because methanol is a very polar solvent
What will happen if there is an overrun of solvent in paper chromatography?
The Rf value cannot be determined since the distance of the solvent may overlay in the paper.
A method of separating mixtures by using chromatographic paper and a solvent?
This method is called paper chromatography.
Is water a major component of the solvent used in paper chromatography?
The water dissolves the paper molecules.
What is meant by tailing a spot in Chromatography?
Chromatography is a method of analyzing the contents of a mixed substance. It is performed by dissolving the substance in a suitable solvent. A drop or spot of the resulting liquid is deposited near the edge of a piece of absorbent paper, such as blotting paper or special chromatography paper. The strip of paper with the spot at the bottom is then suspended over a solvent with the bottom edge of the paper in the solvent. As the solvent soaks upwards, the solvent carries some particles upwards and away from the spot. Different compounds within the mixture will travel at different rates and eventually the strip of paper will show different bands of colors, separating out and indicating some of the various components that were in the original substance. 'Trailing a spot' is a short term for this laboratory process.
A hypothesis for paper chromatography?
A hypothesis for paper chromatography depends on what you are making the hypothesis on. A hypothesis for the speed of chromatography could be that you think the speed of the process can be changed depending on the type of paper, or whatever the stationary phase is, and the type of solvent being used.
What is paper chromatograph?
Ascending paper chromatography is where a chromatogram rises, or ascends. The paper moves up because the solvent moves it in an upward direction.
What is ascending paper chromatograph?
Ascending paper chromatography is where a chromatogram rises, or ascends. The paper moves up because the solvent moves it in an upward direction.
In chromatography why do some colours not spread up the paper?
They do not move up because the aren't soluble the solvent
