What is HPLC and how do you use it?

HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
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Why acetonitrile is used for the calibration of hplc?

In the laboratory, acetonitrile is used as a medium-polarity solvent that is miscible with water and has a convenient liquid range. With a dipole moment of 3.84 D, acetonitril (MORE)

What detectors are used in HPLC?

The detector for an HPLC is the component that emits a response due to the eluting sample compound and subsequently signals a peak on the chromatogram. It is positioned immedi (MORE)

Cooking Using the Scoville Scale

If you've ever cooked with chili peppers, you may have used the Scoville scale to measure how hot they were. This scale employs Scoville Heat Units (SHU) to indicate how much (MORE)
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Why caffeine is used for calibration of HPLC?

Caffeine responds in 3 wavelength regions i.e196 - 208nm in which caffeine maxima at 203±2nm240 - 248nm in which caffeine minima at 244±2nm270 - 278nm in which caffeine maxi (MORE)

How do you use hplc?

  This is a difficult question to answer because there are so many uses for the HPLC. The HPLC is widely used to separate a complex mixture into individual compounds. Sci (MORE)
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Why internal standard you used in hplc method?

Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the sta (MORE)