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What is HPLC and how do you use it?

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HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
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How do you use hplc?

  This is a difficult question to answer because there are so many uses for the HPLC. The HPLC is widely used to separate a complex mixture into individual compounds. Sc

What are the columns used in hplc?

large columns, specifically for packing.  * analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector.  * Narrow bore columns, for more sensiti

Why caffeine is used in hplc calibration?

Caffeine having different wavelents it having 2 maximas  and 1 minima.  1st maxima is 205nm  2nd maxima is 273nm  minima is 245nm  and it is primary reference standa

Why acetone used in hplc calibration?

UV cut off of acetone is 330 nm and it is easyly available,non expensive. you can use any other solvent which has UV cut off above 250.

Who uses a hplc?

Analytical chemists and Polymer Chemists

Why caffeine is used for calibration of HPLC?

Caffeine responds in 3 wavelength regions i.e 196 - 208nm in which caffeine maxima at 203±2nm 240 - 248nm in which caffeine minima at 244±2nm 270 - 278nm in which caffeine m

Why acetonitrile is used for the calibration of hplc?

In the laboratory, acetonitrile is used as a medium-polarity solvent that is miscible with water and has a convenient liquid range. With a dipole moment of 3.84 D, acetonitril