What is HPLC and how do you use it?

HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
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Why acetonitrile is used for the calibration of hplc?

In the laboratory, acetonitrile is used as a medium-polarity solvent that is miscible with water and has a convenient liquid range. With a dipole moment of 3.84 D, acetonitril (MORE)

What detectors are used in HPLC?

The detector for an HPLC is the component that emits a response due to the eluting sample compound and subsequently signals a peak on the chromatogram. It is positioned immedi (MORE)
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Why are you using caffeine in hplc calibration?

caffein is a highly stable, this is show dual lamda max like 205 and 275 nm, it is not a degradable. this is cheep chemical.
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Why caffeine is used for calibration of HPLC?

Caffeine responds in 3 wavelength regions i.e196 - 208nm in which caffeine maxima at 203±2nm240 - 248nm in which caffeine minima at 244±2nm270 - 278nm in which caffeine maxi (MORE)

How do you use hplc?

  This is a difficult question to answer because there are so many uses for the HPLC. The HPLC is widely used to separate a complex mixture into individual compounds. Sci (MORE)
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Why internal standard you used in hplc method?

Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the sta (MORE)