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What is HPLC and how do you use it?

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HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
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Why acetonitrile is used for the calibration of hplc?

In the laboratory, acetonitrile is used as a medium-polarity solvent that is miscible with water and has a convenient liquid range. With a dipole moment of 3.84 D, acetonitril

What detectors are used in HPLC?

The detector for an HPLC is the component that emits a response due to the eluting sample compound and subsequently signals a peak on the chromatogram. It is positioned immedi

Why are you using caffeine in hplc calibration?

caffein is a highly stable, this is show dual lamda max like 205 and 275 nm, it is not a degradable. this is cheep chemical.

Why use caffeine for hplc calibration?

Caffeineis dule absorbance,it gives multiwavelength responds like 205,245,273.

Why caffeine is used in hplc calibration?

Caffeine having different wavelents it having 2 maximas  and 1 minima.  1st maxima is 205nm  2nd maxima is 273nm  minima is 245nm  and it is primary reference standa

Why caffeine is used for calibration of HPLC?

Caffeine responds in 3 wavelength regions i.e 196 - 208nm in which caffeine maxima at 203±2nm 240 - 248nm in which caffeine minima at 244±2nm 270 - 278nm in which caffeine m

How do you use hplc?

  This is a difficult question to answer because there are so many uses for the HPLC. The HPLC is widely used to separate a complex mixture into individual compounds. Sc

Why internal standard you used in hplc method?

Internal standard can be used for calibration by plotting the ratio of the analyte signal to the internal standard signal as a function of the analyte concentration of the sta

How HPLC is used as separation technique?

it used a mobile phase to carry a sample through a tube containing a stationary phase which is a liquid coated on solid particles.

Why caffeine is used in calibration of hplc?

it is retained to a lesser extent....has dual wavelength detection (245nm, 350nm) and gives a sharp and consistent peak.

What is hplc?

high performance liquid chromatography. Generally the stationary phase in HPLC is known as reversed phase as the stationary phase is non-polar while the mobile phase is polar.
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What are the columns used in hplc?

large columns, specifically for packing.  * analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector.  * Narrow bore columns, for more sensiti
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Why caffeine solution using for hplc calibration?

It is very stable(3 days at room temp.) ,it is having multi wavelength like 2 maxi ma (205 ,273 nm and minima(245nm) and cheap cost.

Why is propylparaben is used in calibration of HPLC?

propyl paraben being a stable compound with reproducible chromatographic parameters used as a internal standard for the calibration of the HPLC systems