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What is HPLC and how do you use it?

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HPLC stands for high performance liquid chromatography. It is a liquid chromatography which involves the separation of the compounds on the basis of their polarity. It is used to analyze, identify, purify & quantify the compounds.
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How do you use hplc?

This is a difficult question to answer because there are so many uses for the HPLC. The HPLC is widely used to separate a complex mixture into individual compounds. Scienti

What are the columns used in hplc?

large columns, specifically for packing. . analytical columns, for quantitative analysis, usually accompanied by a UV-vis detector. . Narrow bore columns, for more sensitive

What detectors are used in HPLC?

The detector for an HPLC is the component that emits a response due to the eluting sample compound and subsequently signals a peak on the chromatogram. It is positioned immedi

Why caffeine is used in hplc calibration?

Caffeine having different wavelents it having 2 maximasand 1 minima.1st maxima is 205nm2nd maxima is 273nmminima is 245nmand it is primary reference standard and also suggeste

Why acetone used in hplc calibration?

UV cut off of acetone is 330 nm and it is easyly available,non expensive. you can use any other solvent which has UV cut off above 250.

Who uses a hplc?

Analytical chemists and Polymer Chemists
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Why caffeine is used for calibration of HPLC?

Caffeine responds in 3 wavelength regions i.e 196 - 208nm in which caffeine maxima at 203±2nm 240 - 248nm in which caffeine minima at 244±2nm 270 - 278nm in which caf

Why acetonitrile is used for the calibration of hplc?

In the laboratory, acetonitrile is used as a medium-polarity solvent that is miscible with water and has a convenient liquid range. With a dipole moment of 3.84 D, acetonitril