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A technique where a long chain of same (homo) nucleotides (polymer) are attached to the 3' OH end. This procedure is very useful for subcloning :
(Suppose the one in bold is the target gene and the other is your vector)
5'------------- AA(An)AA-OH --------------3'
+
3'------------- OH-TT(Tn)TT--------------5'
|
|DNA Ligase permanently joins the fragments together
|
V
5'------------- AA(An)AA------------- 3'
3'--------------TT(Tn)TT--------------5'
The bad part is you can't control the length.
Look up Terminal transferases for more details.
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