0
gas chromatographt (GC) and High Performance Liquid Chromatography (HPLC) are different , and to understand why you must think about what chromatography is:
Chromatography in its simplest form is like putting ink on blotting paper and watching the colours separate.
Liquid chromatoraphy uses a "column" which is made from bare or bonded silica, it separates a mixture of compounds by how polar they are. You can use a gradient of different solvents.
GC also uses a column, but it is a capillary column and instead of using a liquid to carry your mixture which needs to be separated it uses a carrier gas, like nitrogen. You can vary the temperatures in both LC and GC to aid better resolution.
GC is used for more volatile compounds and LC is used more less volatile. HPLC usually refers to reversed phase, normal phase is where the column is vare silica which is very polar. Bonded silica is bonded with hydrocarbons which is non polar.
The thing to remember is that "like attracts like" so if the column in non polar, the compound to elute first will be the most polar.
To summarise, they are both separation techniques, one uses gas and the other liquid. You would choose which one to uese depending on how volatile the compounds which you want to separate are.
Vishal Bobade NCL,Pune
Wiki User
∙ 15y ago
Wiki User
∙ 10y agoHPLC (high performance liquid chromatography) is different to FPLC (fast protein liquid chromatography) in terms of pressure. FPLC usually have a maximum pressure of 4MPa, whilst HPLC can cope with much higher pressures.
Wiki User
∙ 12y agoRRLC(RAPID RESOLUTION LIQUID CHROMATOGRAPHY) is a variant of HPLC using columns with particle size <2 um (typically, 1.8 um), which provides significantly better separation than the traditional (5 um) columns and enables much faster analysis. Strictly speaking, "RRLC" is AGILENT Corporation trademark, but is often used as a name for the technique in general.
Wiki User
∙ 11y agoI do not know what either of those things are but I bet there is a difference just letting you know that I think that HPLC stand for HEY.PLEASE.LICK.COCK. And GC stands for GO.CUNK
Add your answer:
Earn +20 pts
What is difference between specificity and selectivity in hplc method validation?
specificity and selectivity
What is difference between isocratic and gradient hplc?
in isocretic system we use only single mobile phase but in gredent system we use two or more mobile phases.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
Retention time in Hplc?
The Time-Taken the sample Or elute in the column is called the retention time in hplc.
Why volatile compounds are analysed in gc but not in hplc?
GC can give very resolved sharp peaks with short run time compared to hplc. additionally, there is less compatibility issue in setting an MS up to a GC than HPLC
What is Difference Between HPLC UV detector Spectrophotometer uv detector?
hi, could any one answer what is the difference between hplc uv detector and spectrophotometer uv detector ? my id is debendra_mallick2002@yahoomail.com
What is difference between specificity and selectivity in hplc method validation?
specificity and selectivity
What is difference between an HPLC UV detector and a fluorescence detector?
flourescence is more sensetive than UV detection
What is difference bet GLC and HPLC?
GLC has a stationary liquid phase and gas moving phase HPLC had a stationary solid phase and liquid moving phase HPLC is done under high pressure. HPLC can be used for thermally unstable compounds as opposed to GLC HPLC can be used for polar or low volatile compounds as opposed to GLC
What is difference between isocratic and gradient hplc?
in isocretic system we use only single mobile phase but in gredent system we use two or more mobile phases.
What is the deference between GC and GCHS?
What is deference GC, HPLC & GCHS
How do you distinguised np-hplc and rp-hplc?
NP-HPLC is "Normal Phase" HPLC, wherein the solvents used are less polar than the substrate in the HPLC column (e.g. using hexane or dichloromethane with a silica HPLC column). RP-HPLC is "Reverse-Phase" HPLC, wherein the solvents used are more polar than the substrate in the HPLC column (e.g. using Water and Methanol with a octadecylsilane (ODS or C18) column).
Which one is advanced gc or hplc?
HPLC is advanced
Retention time calculation for hplc?
why RT was shifting & how to RT calculation in HPLC
What is C8 and C18?
HPLC columns. (HPLC - High Performance Liquid Chromatography.)
Definition of standard in HPLC?
Answer: HPLC standards are an indispensible tool for analytical HPLC applications. They are used to monitor column performance & calibrate detector response.
What type of species can be separated by HPLC but not by gas liquid chromatography?
mixture of enantiomers can be separated by HPLC
