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What is the methodology for producing recombinant DNA to be used in gene cloning?
Wiki User
∙ 10y ago
1. A vector such as plasmid is needed along with a host cell. Restriction enzymes and DNA ligase are enzymes that are used to introduce foreign DNA into a vector.
Wiki User
∙ 10y ago
Wiki User
∙ 11y agoStep 1: The specified gene is inserted into a plasmid.
Step 2: The plasmid is put into a bacterial cell.
Step 3: The host cell is grown in a culture to form a clone of cells containing the "cloned" gene of interest.
Step 4: Basic research is acquired and various applications applied.
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The cloning of many genes in hopes that at least one of the clonies of clones will contain the desired gene is referred to as the?
recombinant clone or chimeric DNA
Definition of gene cloning?
Gene cloning is the replication of DNA fragments by the use of a self-replicating genetic material. Unlike reproductive cloning, which replicates an entire organism, gene cloning duplicates only individual genes of an organism's DNA.
What is gene cloning and gene modification?
Gene cloning is when you take the entire genetic profile to create a copy of the donor of the genes. Gene modification is when you change a persons genes, in a way to change the outcome of how the baby will look, act, her/his personality and a lot more. In this case, gene cloning would come under the category of gene manipulation.
The process of using plasmids gene cloning?
That's right! :P
Where is the multiple cloning site present in pUC 18?
It is within the LacZ' gene.
What is the role of Cloning Host in recombinant DNA technology?
The role of cloning host in RDT is to serve as the host cell under culture, in which the designed gene is subjected to produce recombinant protein.
In the process of human gene cloning using recombinant plasmids what is the bacterial plasmid?
functions as a vector
Explain the role cloning vectors play in making recombinant DNA?
they carry a gene like a vehicle
What step is not essential in producing recombinant DNA?
The step is not essential in producing recombinant DNA is reading the sequence of nucleotides of the particular gene being altered. Cutting the gene from the others is an essential step.
True or false gene cloning is an efficient means of producing large numbers of genes?
True, gene cloning, is an efficient way of producing large numbers a gene. These genes can be cloned repeatedly, at a rather as a rather efficient means.
Why is cloning an example of cloning?
Gene Cloning is used to clone a gene of interest in a vector called plasmid. The chimeric DNA or rDNA formed by cloning is stable and can be used to propagate and sequence the DNA. producing vector containing inulin gene is an example.
Why is gene cloning an efficient method of producing a substance such as interferon artificially?
Yes
The cloning of many genes in hopes that at least one of the clonies of clones will contain the desired gene is referred to as the?
recombinant clone or chimeric DNA
List the four distinct stages involved in a gene transfer?
1.Cleaving DNA The source chromosomes is cut into fragments of DNA.2.Producing recombinant DNA The DNA fragments containing the desired gene are inserted into viral or bacterial DNA. The recombinant DNA is then allowed to infect the target cells.3.Cloning cells infected cells are allowed to reproduce.Growing a large number of identical cells from one cell is known as cloning.4.Screening target cells Targets cells that have received the particular gene of interest are isolated.
What results from inserting foreign DNA into an organism to produce a new gene combination?
I don't know whoever posted the answer before this (Gene Cloning) but it's wrong - the correct answer would be Recombinant DNA.
What are the four steps required for DNA recombination?
The four steps of genetic engineering are: Isolating Gene- isolate pieces of DNA with the desirable gene Cutting the desired gene - Removing gene from the organism. Making Recombinant DNA- placing the small pieces of DNA into a host cell. Cloning and Screening- for colonies that are producing the desired protein
How does recombinant DNA help cure diseases like diabetes?
recombinant dna has a foriegn gene that contains the coded information for the preparation of proper gene which is then placed instead of mutated gene........
