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Oligonucleotide are single-strand and short DNA. Oligonucleotide has a use in the areas of forensics, genetic testing and research in the field of science.
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What is the principle of allele specific oligonucleotide polymerase chain reaction?
Allele specific PCR is used in the process of DNA fingerprinting where specific alleles Can be amplifies and the degree of similarity or difference between individuals can be determined.
What is adapter in recombinant DNA technology?
adapters are smal duble stranded oligonucleotide molecules having a known 3' overhang part which flank the middle double stranded part...when it is phophatase treated it is called modified adapter...rikoganguly@gmail.com
What kind of molecules are transcripts?
DNA Molecules
What kind of macromolecule is DNA polymerase?
DNA polymerase is an enzyme that helps catalyze the polymerization of DNA bases (deoxyribonucleotides) into a DNA strand.
Site directed mutagenesis is a molecular biology method used to make what?
Site directed mutagenesis, also called site-specific mutagenesis or oligonucleotide-directed mutagenesis, is used to make specific, intentional changes to the DNA sequence of a gene and/or gene products. It is used to investigate the structure and biological activity of RNA, DNA, and protein molecules. Also used for protein engineering.
How do you calculate the tmfvalue?
The melting temperature TM, characterises the stability of the DNA hybrid formed between an oligonucleotide and its complementary strand. At TM 50% a given oligonucleotide can hybridised to its complementary strand. By: Zoya Mobeen
What is linker in recombinant DNA technology?
Linkers are synthetic double strsnded oligonucleotide which contain within them one or more Restriction sites that are used to ligate the gene of interest to the vector DNA.
Can you please provide the CAS No for the oligonucleotide i.e. oligotrimedylic acid?
I believe there is no CAS No for oligonucleotide. However, here's the CAS No for DNA and RNA. Hope it will be helpful. Deoxyribonucleic Acid CAS No. 9007-49-2 Ribonucleic Acid CAS No. 66082-38-0
Which of the following is needed in the process of DNA replication?
The ingredients needed for DNA replication include a short oligonucleotide primer and dNTPS. It also needs DNA polymerase and different transcription and translation factors.
What is dharmacon research about?
Dharmacon was founded to research, develop, and market a new technology for RNA oligonucleotide synthesis. RNA include a large family of biological molecules vital in unlocking DNA.
What is the principle of allele specific oligonucleotide polymerase chain reaction?
Allele specific PCR is used in the process of DNA fingerprinting where specific alleles Can be amplifies and the degree of similarity or difference between individuals can be determined.
Why are there nucleotides A T G and C in the master mix and what are the other components and what are there functions?
The nucleotides also called dNTPs are free nucleotides that are used in the replication of new strands. The complete master mix contains Taq DNA polymerase, deoxynucleotides, oligonucleotide primers, magnesium ions, and buffer. Function: Taq DNA-polymerase: Enzyme is heat-resistant as it can withstand the highest temperature during the PCR process. deoxynucleotides (dNTPs): Nucleotides that are used in the replication of new strands. Oligonucleotide primers: Pieces of DNA complementary to the template that tell DNA polymerase exactly where to start making copies. Magnesium ions: A cofactor (catalyst) required by DNA polymerase to create the DNA chain. Salt buffer: Provides the optimum ionic environment and pH for the PCR reaction.
What kind of mixture is DNA?
DNA is not a mixture.
What is adapter in recombinant DNA technology?
adapters are smal duble stranded oligonucleotide molecules having a known 3' overhang part which flank the middle double stranded part...when it is phophatase treated it is called modified adapter...rikoganguly@gmail.com
How many kinds of DNA are there?
There is only one kind of DNA
What kind of molecules are transcripts?
DNA Molecules
What kind of macromolecule is DNA polymerase?
DNA polymerase is an enzyme that helps catalyze the polymerization of DNA bases (deoxyribonucleotides) into a DNA strand.
