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In aqueous formamide solvent systems are particularly useful for preparing

RNA:DNA hybrids, because the hybrids are more stable than DNA:DNA duplexes

in these solvents.

It is therefore possible to find conditions under which incubation of RNA with DNA duplexes leads to the formation of R-loops and conditions under which RNA:DNA hybrids will form from the respective single strands but DNA:DNA duplexes are unstable and do not form.

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Q: Is DNA-RNA hybrid is more stronger than RNA-RNA hybrid?
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