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Why pH of Soyabean Casein Digest Medium should be adjusted to 7.3?
Wiki User
∙ 16y ago
The maximal growth pH ranges for bacteria are well defined hence to make a medium which meets the requirements of most bacteria a neutral pH is used. 7.3+-0.2 This pH allows for most organisms to survive even though this pH may not allow for optimized growth. Hence it is at this pH to allow relatively good growth of different microbes.
Wiki User
∙ 16y ago
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Why Soybean Casein Digest Medium is used for sterility purpose?
Soyabean Casein Digest Medium is a very good media for bacteria growth. A little amount of bacteria can grow further in this media so it is used in Sterility Purpose to check the presence of bacteria in a Solution.---Subhajit NaskarB.Pharm. Jadavpur University
Why must use starch casein nitrate agar and what is the starch casein agar properties?
Because it is a selective medium
What was the medium used by sci-fi artist John Berkey?
Casein paint!
What is the chemical composition of fluid thioglycollate medium?
Enzymatic Digest of Casein 15 g,Yeast Extract 5 g,Dextrose 5.5 g, L-Cystine 0.5 g,Sodium Chloride 2.5 g,Sodium Thioglycollate 0.5 g,Resazurin 0.001 g, Agar 0.75 g Final pH: 7.1 ± 0.2 at 25°C Formula may be adjusted and/or supplemented as required to meet performance specifications
How do you sterilize starch casein agar medium?
Dear Friend You can autoclave this medium but be carefull about volume of media because in time of heating it overflows. I make about 300 cc medium in a 1L erlenmeyer.
What has the author Dilys Henry written?
Dilys Henry has written: 'The corrosion resistance of some stainless steels in a milk derived medium, acid casein whey'
Why is Tryptic soy agar considered a complex medium?
It's complex because the constituent elements of the medium (enzymatic digests of casein and soybean) are present in an undefined amount, and an amount which will vary from batch to batch. Contrast this to a chemically defined medium which will always contain the exact amount of constituent.
Does HCI digest protein?
No,a digestive enzyme known as 'Pepsin' digests proteins.But yes,HCl is an acidic medium for 'Pepsin to work.Without HCl,our body cannot digest proteins.
What ingredient in PEA supplies nitrogen?
PEA agar, also referred to as Phenylethyl Alcohol Agar is a medium used by microbiologists to grow microorganisms. The ingredient that supplies nitrogen is casein.
Why must a powdered complex medium be sterilized immediately after being stored for months?
Because the microbes will start to digest it almost immediately, and their products will change the medium properties.
Why is litmus milk a good differential medium?
Litmus milk contains several components such as; lactose (milk sugar) casein (milk protein) and Litmus ( Ph indicator) that can be metabolized. Thats why it is considered a good differential medium
What is litmus milk used for?
Litmus milk is an undefined medium consisting of skim milk and the pH indicator azolitmin. It is used to differentiate members within the genus Clostridium. It differentiates Enterobacteriaceae from other Gram-negative bacilli based on enterics' ability to reduce limus. The skim milk provides nutients for growth. The protein is casein and the lactose is for fermentation. Azolitmin is purple between pH of 4.6 and 8.2. It turns pink when pH reaches 4.5 and blue at a pH of 8.3. Litmus milk has four major reactions: lactose fermentation, reduction of litmus, casein coagulation, and casein hydrolysis. Lactose ferminatation turns the litmus pink due to acidity. If litmus is reduced during lactose fermentation it will turn the medium white. Acid may cause an acid clot this is due to the casein precipitating. Heavy gas production can cause stormy fermentation, this is due to the breaking up of the clot. Protelolytic enzymes such as chymotrypsin , rennin, and pepsin produced by some bacteria produce a curd. A curd differs from an acid clot since it will not dissolve in alkaline conditions. In those conditions the curd will tend to retract from the sides of the tube revealing a straw-colored fluid called whey. A digestion reaction leaves only a clear to brownish fluid. This is due to certain caseases digesting both acid clots and curds. Bacteria that only partially digest the casein produce ammonia raising the pH turning the litmus blue indicating an alkaline reaction. This can be either a blue or purple ring at the top of the clear fluid or bluing of the entire medium.
