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Enzymatic Digest of Casein 15 g,Yeast Extract 5 g,Dextrose 5.5 g, L-Cystine 0.5 g,Sodium Chloride

2.5 g,Sodium Thioglycollate 0.5 g,Resazurin 0.001 g, Agar 0.75 g

Final pH: 7.1 ± 0.2 at 25°C

Formula may be

adjusted and/or supplemented as required to meet performance specifications

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Related Questions

What does a blue or pink color in thioglycollate mean?

A blue color in thioglycollate indicates the presence of oxygen in the growth medium, as oxygenated environments turn the indicator dye blue. In contrast, a pink color signifies that the medium is anaerobic, as the dye is reduced in the absence of oxygen, turning pink.


Why you use Fluid Thioglycollate medium for bacterial contamination checking?

Fluid Thioglycollate is a medium used to grow and observe all manner of aerotolerance in bacteria. Therefore if you were to place a sample in it to see if it contained bacteria or not, given the correct temperature needed, the bacteria would be able to be observed fairly quickly as they would grow exponentially in this nutrient rich environment.


Why use fresh thioglycollate medium?

A fluid thioglycollate is preferably used soon after preparation to minimize the amount of oxygen that is permitted to diffuse towards the bottom of the medium. The longer you wait before using this medium, the more oxygen is allowed to diffuse into it; more oxygen will negatively affect the growth of obligate anaerobes but would positively affect the growth of aerobic organisms.


Is a thick band or thin band more desirable at the surface of Fluid Thioglycollate?

A thin band is generally more desirable at the surface of Fluid Thioglycollate medium, as it indicates a higher concentration of aerobic organisms that are more likely to grow in oxygen-rich conditions. A thick band may suggest overgrowth or contamination, which can complicate the interpretation of microbial growth. The thin band allows for clearer differentiation of microbial growth patterns and better assessment of the organisms present.


Where would you expect to see growth of a strict aerobe anaerobe microphile and facultative anaerobe in the fluid thioglycollate medium?

The sodium thioglycollate in the broth creates a redox potential in the tube, with higher levels of oxygen at the top of the tube, and a complete absence of oxygen at the bottom of the tube.Fluid thioglycollate broth also typically contains a redox potential indicator such resazurin, which produces a pink color in an oxidized environment. As with the BHI media, organisms will only be able to grow where their oxygen requirements are met, and will localize to the area(s) of their oxygen requirements in the fluid thioglycollate broth.Obligate aerobes will grown on or very close to the top (high oxygen). Obligate anaerobes will only grow on the bottom (no oxygen). Facultative anaerobes will grow throughout but more on top. Microaerophiles will be found in a band where only a small amount of oxygen is found. They would be close to but not on the top.


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Why is there a colored band at the surface of fluid thioglycollate medium which is more desirable a thick or thin colored band?

The higher viscosity of the Fluid Thioglycollate Medium prevents rapid uptake of oxygen and helps maintain the stratification of organisms growing in different layers of the broth.Oxygen is driven out of the broth by autoclaving, but as the broths sit at room temperature, oxygen begins to diffuse back into the tube. Any increase in the oxygen content is indicated by the redox indicator sodium resazurin which changes its color to red.Obligate aerobes will only grow in this oxygen-rich top layer. Obligate anaerobes will only grow in the lower areas of the tube. Microaerophiles will grow in a thin layer below the richly-oxygenated layer. Facultative or aerotolerant anaerobes can grow throughout the medium but will primarily grow in the middle of the tube, between the oxygen-rich and oxygen-free zones.


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