Crystal Violet is a dye that accumulates in the cell nucleus.
The fixed dye, measured photometrically after solubilization,
correlates with the nuclear DNA content and thus with cell
number.
Crystal Violet is a dye that accumulates in the cell nucleus.
The fixed dye, measured photometrically after solubilization,
correlates with the nuclear DNA content and thus with cell
number.
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Blood and Drabkins Reagent (RBCs lysed and Hgb released)
Hgb oxidized to methoglobin by K ferricyanide
Resulting pigment converted to stable cyanmethoglobin by
Kcyanide
Absorption of pigment measured photometrically at 540nm
Concentration of Hgb is directly proportional to optical density
of solution