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Why are contrasting colors important in gram staining?
# Suppose you performed a gram stain on a sample from a pure culture of bacteria and observed a friend of red and purple cocci. Adjacent were not always the same color. What do you conclude? # ## The bacteria was not a pure culture.
When bacteria culture age does it appear gram negative or gram positive?
Typically, a negative result can be doubted, but a positive staining cannot be. This is because the vast majority of bacteria that do revert (not to say that the majority of bacteria DO revert) will go from positive to negative. This is due to a decreased average level of peptidoglycan synthesis in Agar culture, as opposed to natural function in vivo. The decreased level of peptidoglycan allows the ethanol/acetone decolorizer to more quickly flush the Crystal Violet from Gram+ cells, making them appear either weakly positive, or fully negative.
How does culture age affect results of a spore stain?
The age of the culture used for a spore stain can impact the results by affecting the viability and sporulation of the organism. A young culture with actively growing cells is more likely to produce good spore stain results, while an older culture with decreased viability and sporulation may lead to unreliable staining outcomes. It is generally recommended to use a fresh culture for spore staining to obtain accurate and reliable results.
What are some of the cytological techniques?
Some cytological techniques include microscopy, cell staining, immunofluorescence, flow cytometry, and cell culture. These techniques are used to study the structure, function, and behavior of cells at a microscopic level.
What might cause a Gram variable reaction?
A Gram-variable reaction may occur when the bacteria have an atypical cell wall structure, making it difficult to predict whether they will appear as Gram-positive or Gram-negative under a microscope. This variability in staining can be caused by factors such as age of the culture, cell wall composition, or bacterial species.
Why use citric acid in crystal violet staining?
Crystal Violet staining is a technique used in cell culture laboratories to determine concentration of cells in culture liquid. As against other means of cell staining and counting (e.g. Trypan blue) the entities counted using the Crystal Violet staining are the cell nuclei instead of whole cells. This technique has the advantage that also concentrations of aggregated cells can be determined accurately. Here comes the Citric acid in. Nuclei are situated within the cell. To extract the nuclei out of the cells Citric acid is used to make the cell burst so that the Crystal Violet can stain the nuleus and thus concentration of cells can be determined using a microscope and a counting chamber.
Why are contrasting colors important in gram staining?
# Suppose you performed a gram stain on a sample from a pure culture of bacteria and observed a friend of red and purple cocci. Adjacent were not always the same color. What do you conclude? # ## The bacteria was not a pure culture.
What is the principle of orbital shaker?
The principle of an orbital shaker is to create a smooth, continuous motion by shaking samples in a circular motion. This motion helps to mix, agitate, or incubate samples in a controlled environment. Orbital shakers are commonly used in laboratories for various applications such as cell culture, solubility studies, and staining procedures.
What was the principal focus of Greed culture?
The Greed Culture was a principle founded by Alexander the Great. The principle focus of the Greed culture was acceptance of the cultures of the lands and people that Alexander the Great conquered.
Which principle of coin operations identifies the need to learn the language and culture of the people?
The principle that identifies the need to learn the language and culture of the people is the Principle of Local Presence. This principle maintains that in order to successfully carry out coin operations in a foreign country, understanding the local language and culture is crucial for building trust and creating effective relationships with the local population.
When bacteria culture age does it appear gram negative or gram positive?
Typically, a negative result can be doubted, but a positive staining cannot be. This is because the vast majority of bacteria that do revert (not to say that the majority of bacteria DO revert) will go from positive to negative. This is due to a decreased average level of peptidoglycan synthesis in Agar culture, as opposed to natural function in vivo. The decreased level of peptidoglycan allows the ethanol/acetone decolorizer to more quickly flush the Crystal Violet from Gram+ cells, making them appear either weakly positive, or fully negative.
Why is it necessary to use a young culture 24-hr old culture in gram staining?
less than 24 hours old. Older cultures tend to lose the ability to retain stains.
What is the principle that groups of people with shared characteristics such as language and culture should be allowed to control their own governments?
The principle is self-determination.
What is the importance of developing pure microbial culture?
This is important in order to classify, characterize and diagnose the disease the culture may cause. This will help in staining, and also further the identification and specification of the bactera.
How does culture age affect results of a spore stain?
The age of the culture used for a spore stain can impact the results by affecting the viability and sporulation of the organism. A young culture with actively growing cells is more likely to produce good spore stain results, while an older culture with decreased viability and sporulation may lead to unreliable staining outcomes. It is generally recommended to use a fresh culture for spore staining to obtain accurate and reliable results.
Who is considered responsible for having introduced the principle of talion to Mesopotamian culture?
Hammurabi
What are the directive principle and policy of the Nigerian government on culture the mass media national ethnic and duties of the citizen?
Discuss thew directive principles and policy of the Nigerian Government on culture
