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What is the principle of crystal violet staining in monolayer culture?
Wiki User
∙ 11y ago
Crystal Violet is a dye that accumulates in the cell nucleus. The fixed dye, measured photometrically after solubilization, correlates with the nuclear DNA content and thus with cell number.
Wiki User
∙ 11y ago
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Would it be useful to perform a gram stain on a mixed culture?
The purity of a culture of bacteria is important so it can test on that one type of bacteria. Gram staining can be good so you make sure everything in the streak plate is one color showing that it is gram positive and gram negative.
When bacteria culture age does it appear gram negative or gram positive?
Typically, a negative result can be doubted, but a positive staining cannot be. This is because the vast majority of bacteria that do revert (not to say that the majority of bacteria DO revert) will go from positive to negative. This is due to a decreased average level of peptidoglycan synthesis in Agar culture, as opposed to natural function in vivo. The decreased level of peptidoglycan allows the ethanol/acetone decolorizer to more quickly flush the Crystal Violet from Gram+ cells, making them appear either weakly positive, or fully negative.
What is purity testing of microorganisms?
purity testing of microorganism is to check weahter the microbial culture contain one type of cells of species or it get contaminated or mixed with other type.the best way to check this is by sub culturing and staining.
Why are contrasting colors important in gram staining?
# Suppose you performed a gram stain on a sample from a pure culture of bacteria and observed a friend of red and purple cocci. Adjacent were not always the same color. What do you conclude? # ## The bacteria was not a pure culture.
Can a Pure culture prepared from mixed broth or mixed agar slant?
To identify the pure culture the best method is to perform sub culturing and it can be done either by on another agar plate by streak plate method or by pour plate methodThe next is to perform staining which give the difference in morphology if the culture is not pure. But we should perform both the test and then should corelate them because none of them is individually 100% full proof
Why use citric acid in crystal violet staining?
Crystal Violet staining is a technique used in cell culture laboratories to determine concentration of cells in culture liquid. As against other means of cell staining and counting (e.g. Trypan blue) the entities counted using the Crystal Violet staining are the cell nuclei instead of whole cells. This technique has the advantage that also concentrations of aggregated cells can be determined accurately. Here comes the Citric acid in. Nuclei are situated within the cell. To extract the nuclei out of the cells Citric acid is used to make the cell burst so that the Crystal Violet can stain the nuleus and thus concentration of cells can be determined using a microscope and a counting chamber.
What was the principal focus of Greed culture?
The Greed Culture was a principle founded by Alexander the Great. The principle focus of the Greed culture was acceptance of the cultures of the lands and people that Alexander the Great conquered.
What is the importance of developing pure microbial culture?
This is important in order to classify, characterize and diagnose the disease the culture may cause. This will help in staining, and also further the identification and specification of the bactera.
What is the principle that groups of people with shared characteristics such as language and culture should be allowed to control their own governments?
The principle is self-determination.
Why is it necessary to use a young culture 24-hr old culture in gram staining?
less than 24 hours old. Older cultures tend to lose the ability to retain stains.
Who is considered responsible for having introduced the principle of talion to Mesopotamian culture?
Hammurabi
Does the age of the culture affect the gram staining reaction?
Yes, the age of the culture may influence the results of the stain. With the current theory behind gram staining, it is thought that in gram-positive bacteria, the crystal violet and iodine combine to form a larger molecule that precipitates out within the cell. The alcohol/acetone mixture then causes dehydration of the multilayered peptidoglycan in the gram-positive call wall, thus decreasing the space between the molecules and causing the cell wall to trap the crystal violet-iodine complex within the cell. In the case of gram-negative bacteria, the alcohol/acetone mixture, being a lipid solvent, dissolves the outer membrane of the gram-negative cell wall (and may also damage the cytoplasmic membrane to which the peptidoglycan is attached). The thin layer of peptidoglycan is unable to retain the crystal violet-iodine complex and the cell is decolorized. It is important to note that gram-positivity (the ability to retain the purple crystal violet-iodine complex) is not an all-or-nothing phenomenon but a matter of degree. There are several factors that could result in a gram-positive organism staining gram-negatively: 1. The method and techniques used. Overheating during heat fixation, over decolorization with alcohol, and even too much washing with water between steps may result in gram-positive bacteria losing the crystal violet-iodine complex. 2. The age of the culture. Cultures more than 24 hours old may lose their ability to retain the crystal violet-iodine complex. 3. The organism itself. Some gram-positive bacteria are more able to retain the crystal violet-iodine complex than others.
What are the directive principle and policy of the Nigerian government on culture the mass media national ethnic and duties of the citizen?
Discuss thew directive principles and policy of the Nigerian Government on culture
What is the central sociological principle of education as it relates to a nation's needs?
It is a reflection of the nations culture
Which principle of coin operations identifies the need to learn the language and culture of the people?
The principle that identifies the need to learn the language and culture of the people is the Principle of Local Presence. This principle maintains that in order to successfully carry out coin operations in a foreign country, understanding the local language and culture is crucial for building trust and creating effective relationships with the local population.
What forms the foundation of American culture?
The foundation of American culture is formed by freedom of choice. America was founded on the principle of religious freedom and this concept has leaked over into other forms of culture including entertainment, politics, and architecture.
Does the age of the culture affect the gram stain?
Yes, the age of the culture may influence the results of the stain. With the current theory behind gram staining, it is thought that in gram-positive bacteria, the crystal violet and iodine combine to form a larger molecule that precipitates out within the cell. The alcohol/acetone mixture then causes dehydration of the multilayered peptidoglycan in the gram-positive call wall, thus decreasing the space between the molecules and causing the cell wall to trap the crystal violet-iodine complex within the cell. In the case of gram-negative bacteria, the alcohol/acetone mixture, being a lipid solvent, dissolves the outer membrane of the gram-negative cell wall (and may also damage the cytoplasmic membrane to which the peptidoglycan is attached). The thin layer of peptidoglycan is unable to retain the crystal violet-iodine complex and the cell is decolorized. It is important to note that gram-positivity (the ability to retain the purple crystal violet-iodine complex) is not an all-or-nothing phenomenon but a matter of degree. There are several factors that could result in a gram-positive organism staining gram-negatively: 1. The method and techniques used. Overheating during heat fixation, over decolorization with alcohol, and even too much washing with water between steps may result in gram-positive bacteria losing the crystal violet-iodine complex. 2. The age of the culture. Cultures more than 24 hours old may lose their ability to retain the crystal violet-iodine complex. 3. The organism itself. Some gram-positive bacteria are more able to retain the crystal violet-iodine complex than others.
