Takes a long time, several weeks or months to create the hybridoma cells.

Also costs a lot of money, and need to house and feed the mammal hosts for a long time while you immunize them

The correct spelling is hybridoma. The definition of hybridoma technology is a technology of forming hybrid cell lines that are called hybridmomas. Anti-body B cells with a myeloma cell are fused to form the cell lines.

Tanya Karns has written:

'The behaviour of hybridoma cells in culture'

The different types of fusion technique used in Hybridoma formatio are:

1. fusion mediated by PEG.

2. fusion mediated by DMSO.

3. Electrofusion

4. Viral Transformation (viral mediated fusion) using EB Virus, Sandai Virus.

In order to produce a monoclonal antibody against follicle stimulating hormone (FSH) a B cell capable of producing antibodies against FSH is fused with a myeloma cell. The resulting hybridoma is propagated, and an antibody produced by the hybridoma harvested. The antibody-producing hybridoma is detected utilising a radioimmunoassay together with immunohistology, and the fused cell hybrid selected is subsequently cloned into individual antibody-producing cell lines. In order to purify FSH impure FSH is passed through the monoclonal antibody against FSH bound to a matrix and the captured FSH is eluted from the matrix.