Lipase is produced in the Pancreas.

There are a few different types of lipases; they can be categorized into preduodenal lipases and pancreatic lipases.

Preduodenal lipases include lingual lipase and gastric lipase. Lingual lipase is secreted and produced by Ebner's glands (a type of minor salivary gland) located in in moat-like structures around the circumvallate papillae on the tongue. Gastric lipase is produced primarily in the fundus and body of the stomach by chief cells.

Pancreatic lipase is secreted and produced by the pancreatic acinar cells and are released by them into the pancreatic ducts. They are contained in zymogen granules and are secreted while still in zymogen granules. Note that there are also other lipolytic enzymes secreted by the pancreas, including colipase.