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Haemagglutination inhibition test Influenza virus has two important surface glycoproteins-the haemagglutinin (HA) and the neuraminidase (NA). Antigenic classification and subtyping of influenza viruses is based on these two glycoproteins. HA plays a key role in virus cell entry by binding to cell surface receptors, which are found also on red blood cells of certain species. Binding to red cells results in haemagglutination, which can be observed as a carpet of agglutinated red cells at the bottom of a tube or microtitre well. In the haemagglutination inhibition test (HIT), antibody directed against the viral haemagglutinins block the virus from binding to the blood cells and thus inhibits the haemagglutination reaction
% Ihibition = diameter of control - diamter of test / diameter of control
This is basically ANTIBIOTIC SENSITIVITY TEST, to test whether the given organism is RESISTANT(no zone of inhibition) or SENSITIVE( zone of inhibition) to the given antibiotic.Zone of Inhibition Testing is a fast, qualitative means to measure the ability of an antimicrobial agent to inhibit the growth of microorganisms.The effectiveness is based upon the size of zone of inhibition,diffusability of antibiotic,size of inoculum,type of media used.example: bacillus organism is inoculated with both PENICILLIN and AMPICILLIN ,zone of inhibition is absent in case of penicillin and present in case of ampicillin, this shows that ampicillin (sensitive) worked effectively when compared to penicillin.
mr. newton's biology test?
competitive inhibition
Myelosuppression is inhibition of the bone marrow.
Zone of inhibition :-It is the area on an agar plate where growth of a control organism is prevented by an antibiotic usually placed on the agar surface. If the test organism is susceptable to the antibiotic, it will not grow where the antibioitic is. Zone of exhibition:-
Competitive Inhibition is a substance that binds to the active site in place of the substance while Non-competitive Inhibition is a substance that binds to a location remote from the active site. (:
Competitive Inhibition is a substance that binds to the active site in place of the substance while Non-competitive Inhibition is a substance that binds to a location remote from the active site. (:
How can you determine whether the zone of inhibition is due to death or the effect of the antibiotics?
When a enzyme is inhibited (many proteins are enzymes), it just means that the enzyme will be reduced in its ability to catalyze a reaction. There are a few types of Inhibition like Competitive Inhibition, Noncompetitive Inhibition, and Irreversible Inhibition.
Inhibition - 1976 is rated/received certificates of: USA:R