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The secondary antibodies can bind to multiple sites on the primary antibody and thus produce a brighter signal since more dyes are brought to a single location.
http://www.bio.davidson.edu/COURSES/genomics/method/IMF.html
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What is the advantages and disadvantages of indirect democracy?
Advantages of indirect democracy include representation of diverse perspectives and the ability of elected officials to make informed decisions on behalf of the population. Disadvantages can include potential for corruption, lack of direct citizen participation in decision-making, and the risk of elected officials prioritizing their own interests over those of the people they serve.
What is the difference between direct fired and indirect fired gas heaters?
With a direct fired system the airflow goes directly over the flame. An indirect fired air heater has a heat exchanger that the air passes over. Indirect units provides heat without contamination from combustion by-products.
What is the difference between direct competition and indirect competition?
Direct competition is a company that offers a product that customers may choose over your product. Indirect competition is a company that offers a substitute good.
What is the Definition of direct income and indirect income?
Money that is received straight to the recipient over a period of time.
Explain the difference between direct characterization and indirect characterization?
Direct characterization is when the author explicitly describes the character's traits and qualities, while indirect characterization reveals the character through their actions, thoughts, and dialogue. Direct characterization tells the reader what the character is like, while indirect characterization allows the reader to infer the character's traits based on their behavior and interactions with others.
What democracy is when the political process in which the people are able to have direct control over the government in making decisions?
Indirect democracy!
How did the French and British differ in the tactics they used to control their empires in Asia?
The British ruled directly over their colonies, while the French did not.
What was direct and indirect colonialism?
Direct colonialism refers to a situation where a foreign power maintains control over a territory through the use of a local administration and military presence. Indirect colonialism involves exerting influence and control over a territory through economic, social, and political means without establishing a significant physical presence.
What is the difference between direct message passing and indirect message passing?
În direct message passing communication, the endpoints (client and server) communicate via direct addressing. This means that the sender knows the exact address (ip for ex) of the receiver. In indirect communication, the link between the sender and the receiver is done via a port instead of direct addresses. The port acts as a mailbox for the messages and the sender doesn't need to know the exact ip of the receiver. The sender simply sends the message via a port and the receiver collects the message from that port. This way of communicating has several advantages over the direct way (for example it allows multipoint and multipath communication)
what are factor that affecting the classification of a cost as direct or indirect?
The classification of a cost as direct or indirect depends on a variety of factors. Traceability: The most important factor in determining whether a cost is considered direct or indirect is the ability to trace it to the production process. Direct costs are those which can be directly traced to the production of goods or services, while indirect costs are those which cannot be traced in this way. Timing: The timing of the cost can also be a factor in determining whether it is classified as direct or indirect. Costs which are incurred before production begins are generally classified as indirect, while those incurred during the production process are typically direct. Relationship to Production Process: The relationship of the cost to the production process can also be a factor in determining whether it is considered direct or indirect. Direct costs are those which are related to the materials or labor used in the production process, while indirect costs are those which are necessary but not directly related to the production process. Controllability: Another factor in the classification of a cost as direct or indirect is the degree of control that the company has over the cost. Direct costs are those which are controllable by the company, while indirect costs are those which are uncontrollable and fixed. Flexibility: The flexibility of the cost can also be a factor in determining whether it is classified as direct or indirect. Direct costs are those which can be adjusted in response to changes in production, while indirect costs are those which remain fixed regardless of changes in production.
What does indirect quotations mean?
He asked, "What is an indirect quotation?"
Difference between direct and indirect supervision?
Indirect SupervisionIndirect supervision is characterized by some form of authority over the work of employees not under direct supervision. In other words, the "supervisor" who provides indirect supervision is responsible for the work, but not for the worker. The descriptions above were written in relation to the employee under direct supervision; the following subsections describe persons with responsibility for exercising indirect supervision.Direct SupervisonThe basic characteristics of direct supervision are the assignment of tasks; the observance, review, and evaluation of performance; the administration of line personnel functions (e.g., selection, discipline, grievances, privileges); and responsibility for the worker, as well as the work. The gradations of direct supervision are described below in terms of supervision received by employees.
