Appropriate nursing care plan for koch's disease?

Answer 1

Step 1: Provide students with Oranges showing symptoms of sour rot disease. Have students look at the rotten tissue with hand lenses or dissecting microscopes. The fungus can often be seen as a thin layer of white, slimy, hyphae and spores on tissue in advanced stages of decay. Use a toothpick or dissecting needle to transfer some of this material to a drop of water on a microscope slide and squash under a coverslip. Students should look for hyphae and spores.

Step 2: Once students have associated the fungus with diseased oranges they can follow the procedure outlined above to isolate the pathogen and grow it in pure culture.

Step 3: About one week later students can inoculate healthy oranges with the fungus they isolated from the diseased fruits. They should observe their inoculated oranges over the next week or so as the sour rot symptoms develop.

Step 4: The pathogen should be isolated from the inoculated oranges and its characteristics should be compared with the fungus isolated in step 2.

This exercise can also be carried out using a variety of other fruit rots caused by pathogenic fungi. The Brown Rot disease of stone fruits, caused by the fungus Monilinia fructicola also works quite well. Although Green Mold of oranges, caused by Penicillium sp. can be used, I avoid it because the fungus produces such an abundance of spores that a dropped petri dish or infected fruit can lead to lots of contamination problems in the lab.