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What are differences in appearances of cytoplasm in stained cell and cytoplasm in unstained cell?
Stained cells are easier to see while unstained cells are more difficult to notice. welcommeee:)
What is the unique chemical component of a Gram Negative cell wall?
peptidoglycan
What color would the G- cell be if not counter stained with safranin?
Purple
What cell structures can be seen in a stain onion cell?
The structure that is seen is the cell wall. This keeps the shape of the cell and is only found in plant cells. The organelles that can be see in a stained onion cells all depends on your microscope. Under a x400 light microscope we could see the cell wall, cell membrane, nucleus and cytoplasm,
Some cells have a darker stained area in the middle of the cell what is the name of this organelle?
golgi
Cite the purpose of following reagents in a differential staining procedure?
To distinguish between acid fast positive and acid fast negative bacteria. Acid fast positive bacteria will stain red to pink color and acid fast negative bacteria will stain blue. Acid fast positive bacteria have mycolic acid in their cell wall, which will stain with carbol fuchsin and not decolorize with acid alcohol. Acid fast negative bacteria do not have mycolic acid in their cell wall, and become decolorize with the acid alcohol. Counterstain of methylene blue needs to be done in order to see the acid fast negative.
What is an azurophil?
An azurophile is a cell which has been readily stained with an azure stain.
Does carbolfuchsin stain acid fast negative cells?
Yes, carbolfuchsin can stain acid-fast negative cells. This red dye can easily get into their thin cell wall lipids due to its solubility.
What cell parts take the stain in?
In a typical cell the cell wall gets stained most. But sometimes even the nucleus gets stained but more specifically the chromatin material in the nucleus.
Is mycolic acid found in gram positive and gram negative cell wall composition?
mycobacterium
What are differences in appearances of cytoplasm in stained cell and cytoplasm in unstained cell?
Stained cells are easier to see while unstained cells are more difficult to notice. welcommeee:)
Why is gram staining not usually applied to eucaryotes?
Gram staining is commonly used on prokaryotic bacteria to determine if their cell wall is gram negative or gram positive. Their cell wall is made of peptidoglycan. It responds well and is stained in certain ways depending on the structure (wether it's gram positive or negative). Animal eukaryotic cells don't even have a cell wall to be stained. In the case of eukaryotic plant cells, that do have cell walls, their cell wall is not made of peptidoglycan. In any case, there is no such thing as gram positive, or gram negative, cell walls in eukaryotes. There would be no point in the test, since it's specifically designed for prokaryotic cells.
Is every part of the cell equally stained?
The nucleolus stains darker. It is part of the nucleus.
What is the unique chemical component of a Gram Negative cell wall?
peptidoglycan
What is the neucleolus?
it is the darkly stained part inside the nucleus inside a cell
What color would the G- cell be if not counter stained with safranin?
Purple
What is the colour of the cell wall of a plant cell?
basically it is colorless, but it takes green color when stained with fast green
