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Is tgge and dgge same thing?
Temperature Gradient Gel Electrophoresis (TGGE) is a refinement of Denaturing Gradient Gel Electrophoresis (DGGE). Both use the same principles.
Is loading dye same with binding dye in electrophoresis?
Yes, loading dye contains a tracking dye (usually bromophenol blue or xylene cyanol FF) that helps to visually track the progress of the DNA/RNA samples as they migrate through the gel during electrophoresis. Binding dye, on the other hand, is used to stabilize and stain nucleic acids in preparation for visualization and is often included in products like loading buffers or staining solutions.
What is the purpose of gel electrophoresis?
The buffer is the medium through which the current flows. In the electrophoresis chamber, the anode and cathode are separated and the gel is placed between them. In order to close the circuit and generate the voltage which causes the migration, the entire chamber is filled with a conductive buffer. It is actually possible to perform electrophoresis without a buffer; however this requires a specially made electrophoresis chamber. In these chambers the electrodes actually contact the top and bottom of the gel eliminating the need for a conductive buffer to close the circuit.SDS PAGE electrophoresis uses buffer not primarily as a conductor but for holding a desired pH, dissipating heat and providing SDS in excess in the case of denaturing gels. A gel would run without a buffer as the gel itself is a conductor but the currents involved would heat it to the point of decomposition. Also the volume of liquid in a gel does not allow for an adequate pH buffering system. Holding a pH is extremely important for reproducibility especially in native gels as the pH can change the charge on the peptide. It is true some gels do not require buffer but these are rare cases like isoelectric focusing.the primary application of the buffer would be to conduct electricity,to form a closed circuit
My iPod Touch keeps turning on but the loading wheel comes up then it turns off and does this all over How can you fix it if possible?
the same thing is happening to my 4th gen. but without the loading wheel
Is it a good thing if your browser caches webpages?
Yes, caching webpages is good thing for a browsers. It speeds up the loading next time you open the same page.
Will two molecules having the same molecular weight and charge have the same mobility in electrophoresis?
yes...!
Function of bromophenolblue in agarose gel electrophoresis?
Bromophenol blue or commasive blue functions as a sample staining dye or DNA staining dye it is mixed with sample before loading the sample in wells. The migration of bromophenol blue is same as of DNA i.e. it carries negative charge and move in same direction of DNA with the speed equals to 200-400bp of DNA.It also prevent backflow of sample in vertical gel electrophoresis as the sample is light from the loading buffer which tends to come back from the well so bromophenol blue prevent the back flow.IUPAC NAME:2,6-dibromo-4-[3-(3,5-dibromo-4-hydroxyphenyl)-1,1-dioxo-3-benzooxathiolyl]phenol.Bromphenol blue does not stain DNA. It is simply a dye that 1) helps you visualise your sample as you load it and 2) migrates (unrelated to the DNA) at a speed that is indeed equivalent to about 200-400bp of DNA, depending on the percentage of gel, giving an indication of how far your samples have run. It also does not prevent "backflow". Usually the buffer which you add to your DNA sample before loading on a gel (ie loading buffer) contains a dye such as bromophenol blue (there are others) and will also contain a dense substance, usually glycerol or ficoll. It is the glycerol or ficoll which due to its density will make the sample more dense than the buffer which the gel is run in, and will prevent it floating out of the well.In order to visualise (stain) the DNA you need an agent such as ethidium bromide or sybr green that intercalates with the DNA (slides between the basepairs) and fluoresces under UV light.Coommassie (not commasive) blue is a dye that will stain proteins (not DNA) but is used after the gel has been run to stain the gel. If you use it with an agarose gel, I'm guessing - having never tried it) you would just simply make a big blue mess and not see anything.
Effect of Diluting a buffer?
The buffer capacity increases as the concentration of the buffer solution increases and is a maximum when the pH is equal to the same value as the pKa of the weak acid in the buffer. A buffer solution is a good buffer in the pH range that is + or - 1 pH unit of the pKa. Beyond that, buffering capacity is minimal.
What is the alternative method to extract cytosolic protein only apart from digitonin lysis buffer because i don't have eit in my lab?
Crude is the alternative method. This is the same thing as EIT.
Does anything have the same pH as water?
With the help of a buffer it is possible.
What is the use of creating more than one windows?
there might be a very slow loading thing and a fun game where you have to keep the slow thing open so it can load but it's boring so you want to play games at the same time.
What is difference between single double and circular buffer?
single buffer : you read and write on the same buffer, can be messy if both reading and writing take place at the same time.double buffer : you read one buffer and you write the other one. When both reading and writing are complete, the buffers are swapped. It solves the problem of simultaneous reading and writing but requires synchronization.circular buffer : this a buffer with two pointer : read and write. If both pointers are equal, the buffer is empty.For each write operation, the write pointer advances and each time data is read back, the read pointer advances. It is circular because when a pointer reaches the end, it wraps back to the beginning.It may be used to implement a queue which allows simultaneous reading and writing without synchronization as long as the buffer is not full.A double buffer is basically a circular buffer of size 2 and a single buffer is basically a circular buffer of size 1.
