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A promoter is a sequence of nitrogenous bases in DNA that signals the enzyme complex "DNA-polymerase" to begin unwinding the gene to transcribe it. A promoter is usually referred to as a "TATA (thymine, adenine, thymine, adenine) box" or a "CAAT (cytosine, adenine, adenine, thymine) box". As DNA polymerase makes its way down a section of DNA and comes across TATA box, or a CAAT box, that is the signal to begin transcription.
Oh, say, six distinct 'elements': there is an upstream enhancer element [sequence] farthest away from the Gene. Closer in is always found 'the promotor sequence' [or element]. The 'transcription start' codon - fMet - marks the beginning of the Gene. The deoxyribonucleotide base sequence provides the genetic information. Fifth, all eukaryotes must deal with introns and exons; and sixth, the transcription termination apparatus appears [manifests itself] in several forms.
The two most important sequences are the expression cassette and the selection marker.The expression cassette contains a promoter and termination sequence, with a multiple cloning site sandwiched in between. It is the section of the plasmid that integrates into the genome of the target organism. The gene of interest is cloned into the multiple cloning site by digesting both plasmid and gene fragment with restriction enzymes that produce complementary ends. Once the plasmid has been transferred into the target organism the promoter sequence determines when the gene is transcribed. Some promoters are always active (Cauliflower Mosaic Virus 35S is most common) and others are developmentally or environmentally regulated, so that the gene is only expressed at specific times. The termination sequence determines where transcription stops.The selection marker is used to determine which organisms are carrying the gene of interest. It usually contains a promoter, resistance gene and termination sequence and forms part of the expression cassette. In most cases antibiotic resistance is used as selection. For example the nptII gene encodes for kanamycin resistance.
Does not always reflect the true sequence of events in time
Insulin is like any other protein. This goes for all proteins: if the aminoacid sequence is the same, the protein will always fold the same way. we do not exactly how this works, how each protein knows exactly how to fold.. not yet. If there are errors in translation or transcription than the protein will not fold correctly. The body has many ways to identify and get rid of these errors, which are quite common. An example that comes to my mind is the PEST sequence, which, if exposed, has a complementarity with lysosoms, and the protein will be hydrolyzed.
A promoter is a sequence of nitrogenous bases in DNA that signals the enzyme complex "DNA-polymerase" to begin unwinding the gene to transcribe it. A promoter is usually referred to as a "TATA (thymine, adenine, thymine, adenine) box" or a "CAAT (cytosine, adenine, adenine, thymine) box". As DNA polymerase makes its way down a section of DNA and comes across TATA box, or a CAAT box, that is the signal to begin transcription.
The Fine Structures are as follows: 1) The Start Codon: Met is specific for one amino acid [Met] yet f-Met uniquely specifies the Start codon.2) There are two kinds of 'upstream promotion' sequences: i) the furthest upstream are called the 'Enhancer sequences', and ii) the control sequences nearer to the Gene are called the 'Promoter sequences'.3) Right beside the Promoter sequence is always found the Operator Sequence: this proffers the attachment for and to the Start Codon. After the Start Codon, the Protein Coding Sequence ensues, followed by Termination sequences.
Oh, say, six distinct 'elements': there is an upstream enhancer element [sequence] farthest away from the Gene. Closer in is always found 'the promotor sequence' [or element]. The 'transcription start' codon - fMet - marks the beginning of the Gene. The deoxyribonucleotide base sequence provides the genetic information. Fifth, all eukaryotes must deal with introns and exons; and sixth, the transcription termination apparatus appears [manifests itself] in several forms.
Always Drink Upstream from the Herd was created on 1995-10-31.
Downstream. The source of a river is always upstream.
The two most important sequences are the expression cassette and the selection marker.The expression cassette contains a promoter and termination sequence, with a multiple cloning site sandwiched in between. It is the section of the plasmid that integrates into the genome of the target organism. The gene of interest is cloned into the multiple cloning site by digesting both plasmid and gene fragment with restriction enzymes that produce complementary ends. Once the plasmid has been transferred into the target organism the promoter sequence determines when the gene is transcribed. Some promoters are always active (Cauliflower Mosaic Virus 35S is most common) and others are developmentally or environmentally regulated, so that the gene is only expressed at specific times. The termination sequence determines where transcription stops.The selection marker is used to determine which organisms are carrying the gene of interest. It usually contains a promoter, resistance gene and termination sequence and forms part of the expression cassette. In most cases antibiotic resistance is used as selection. For example the nptII gene encodes for kanamycin resistance.
Water always flows down a river, so upstream is the opposite direction from the direction in which the water flows.
Any pair of numbers will always form an arithmetic sequence.
Anything with a face should be looking inward and almost never outwards, and with Koi, they ALWAYS go upstream and never downstream.
No. It will change dramatically depending on the amount of precipitation upstream.
The mode statistic is not always unique. For example, look at the following sequence of numbers: 1,2,2,3,4,4,5. Both 2 and 4 are modes for this sequence.
No, fiction is not always in sequence order. Some fiction may be nonlinear, meaning the events are not presented in chronological order. This can be a stylistic choice by the author to create suspense, add complexity to the narrative, or convey specific themes or messages.