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No, she died single (The Virgin Queen)
Although there have been many doubts about her celibacy, there has never been any suggestion that was pregnant at any time.
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Related questions
What religion was queen Elisabeth 1 half-sister?
Catholic
What did queen elisabeth 1 die from?
blood poisoning x
How old was elisabeth 1 when she was queen?
16 years old stupid boy or girl
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Why was the England renaissance movement called the Elizabethan Era?
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What is Elisabeth Sladen's birthday?
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How tall is Elisabeth Broderick?
Elisabeth Broderick is 5' 10 1/2".
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