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It probably depends on what kind of injectable hormones are used and the timing of the injections when the female ferret is in estrus. As long as the vulva has reduced in size, would suggest she has been brought out of estrus. In either an injection of hormones or inducing ovulation by a sterile male, the vulva decreases to approximately one-fourth of the estrus size, it does not regress completely. These are suggestions from two different books on ferrets:

* For female ferrets, injectable hormones are given 10 days or more after the swollen vulva is first noticed induce ovulation and end estrus in about a week. They will not work earlier. About 5 days after the injection, there will be a noticeable reduction in the vulva. The timing of the next heat in unpredictable, depending on many factors including photoperiod, diet, and the duration of the jill's heat before hormones were used. * This involves the injection of 100 USP units of chorionic gonadatropin, which acts like LH to promote ovulation and terminate estrus, used for this purpose. The injections for female ferrets should be given after the vulva reaches full size, usually about two weeks after you first notice that it is beginning to swell. If this hormone injection has induced ovulation, the vulva should begin to decrease in size within about one week, and then continue to get smaller. If after a week the vulva shows no sign of shrinking, a second injection should be given. If ther is still no sign of regression in the size of the vulva the following week, a third injection can be tried.

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Q: Can a female ferret still get pregnant even after she's had the Jill jab?
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