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Can a specimen be viewed under a microscope using the 100x objective without a coverslip?
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The area of the specimen seen when looking through the microscope is the?
It depends on the particular microscope, and the magnification being used. Without these details, this question cannot be answered.
What are the different types of lenses of an optical microscope?
The coarsest classification has the ocular or eyepieceand the objective.There are many designs for both these components of microscopes.The objective lens of a microscope is the one at the bottom near the sample. At its simplest it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes to a focus inside the microscope tube. The objective itself is usually a cylinder containing one or more lenses that are typically made of glass; its function is to collect light from the sample.An eyepiece consists of several "lens elements" in a housing, with a "barrel" on one end. The barrel is shaped to fit in a special opening of the instrument to which it is attached. The image can be focused by moving the eyepiece nearer and further from the objective. Most instruments have a focusing mechanism to allow movement of the shaft in which the eyepiece is mounted, without needing to manipulate the eyepiece directly.See the related links below.
How to use a microscope?
1. When moving your microscope, always carry it with both hands (Figure 1, below). Grasp the arm with one hand and place the other hand under the base for support.2. Turn the revolving nosepiece so that the lowest power objective lens is "clicked" into position (This is also the shortest objective lens).3. Your microscope slide should be prepared with a coverslip or cover glass over the specimen. This will help protect the objective lenses if they touch the slide. Place the microscope slide on the stage and fasten it with the stage clips. You can push down on the back end of the stage clip to open it.4. Look at the objective lens and the stage from the side (Figure 2) and turn the coarse focus knob so that the objective lens moves downward (or the stage, if it moves, goes upward). Move it as far as it will go without touching the slide!5. Now, look through the eyepiece and adjust the illuminator (or mirror) and diaphragm (Figure 3) for the greatest amount of light.6. Slowly turn the coarse adjustment so that the objective lens goes up (away from the slide). Continue until the image comes into focus. Use the fine adjustment, if available, for fine focusing. If you have a microscope with a moving stage, then turn the coarse knob so the stage moves downward or away from the objective lens.7. Move the microscope slide around so that the image is in the center of the field of view and readjust the mirror, illuminator or diaphragm for the clearest image.8. Now, you should be able to change to the next objective lenses with only minimal use of the focusing adjustment. Use the fine adjustment, if available. If you cannot focus on your specimen, repeat steps 4 through 7 with the higher power objective lens in place. Do not allow the objective lens to touch the slide!9. The proper way to use a monocular microscope is to look through the eyepiece with one eye and keep the other eye open (this helps avoid eye strain). If you have to close one eye when looking into the microscope, it's ok. Remember, everything is upside down and backwards. When you move the slide to the right, the image goes to the left!10. Do not touch the glass part of the lenses with your fingers. Use only special lens paper to clean the lenses.11. When finished, raise the tube (or lower the stage), click the low power lens into position and remove the slide.12. Always keep your microscope covered when not in use. Dust is the number 1 enemy!
What is proper ways of handling and manipulation microscope?
Manipulate a MicroscopeInstructions1Set up the microscope on a flat surface. Always carry the microscope using two hands if the microscope must be transported from one area to another. One hand should support the bottom while the other hand is holding the arm. Once in place, remove the any covers and plug in the microscope.2Position the slide onto the stage. Clip it into or under the slide holders. Turn on the light from the illuminator under the stage. Center the slide the best way possible without using any focusing knobs or the eyepiece.3Look through the eyepiece, keeping both eyes open to avoid eye strain, while using the course focus knob to bring the stage and slide closer to the objective, which is the lens that extends below the eyepiece, until the object can be seen through the eyepiece. Use the fine focus knob (which will also be on the side of the microscope) to bring the image into sharper focus.4Center the object being viewed. Change the iris diaphragm under the stage using the level to open and close the iris. Change the amount of light entering the specimen as needed. Changing the amount of light going through the slide may show additional details not seen at higher light levels.5Change to the 10X objective which is the next longest lens by rotating the lens pieces. The object should appear larger and only require using the fine focus knob to bring it into sharper detail. Use the iris diaphragm to change the amount of light entering the slide. Continue to the 40X objective and repeat. Each time the object will appear larger and you will be able to see less of the object at any one time while viewing.6Lower the stage and add one or two drops of immersion oil to the slide. Change the microscope to the 100X objective. Slowly raise the stage using the course focus until the objective is touching the oil. Use the fine focus knob to bring the object into sharp focus. Clean the slide and objective with lens tissue after use.7Return the stage to the lowest level. Change the objective back to the 4X lens. Unplug the machine and replace any covers. Carefully return the microscope using both hands to where it is stored.
Why is the microscope a good invention?
The microscope allows humans to see the world of the microcosms and atoms. Special microscopes allow us to see DNA, Genetic material. and microbes, germs, viruses that are not viewable with the human eye. Without the microscope the medical and scientific world would not have progressed as it has. It has save millions of lives for many decades now. Did you know the microscope was discovered accidentally?
What is the used of the coverslip?
A coverslip in Biology is a small rectangle of glass that is mounted on a slide to be viewed under a microscope. The coverslip protects the specimen, keep it in place and/or make it flat.
Why is it often necessary to use a cover slip when using the microscope?
Coverslip is not placed on a microscope but on the stained specimen on slide . This protects objective lens of microscope from getting stain from a wet mount . It also protects permanent slide .
What is the objective lens used to locate the specimen and the first focus?
When viewing a specimen, you must always use the lower power objective lenses first. Low power lens gives the widest field of view and makes it easier to find the specimen when you look through the microscope. Finding the specimen at high power, without first centering it in the field of view at low power, is nearly impossible.
What is a proper way to add a saltwater solution to the cells without removing the coverslip?
You can add small amounts of water without removing the coverslip. To do this, place a single drop of water on one end of the coverslip and draw the water under the coverslip by placing a piece of paper towel on the opposite end of the coverslip. :D
What does the phase microscope do?
A phase microscope is also a contrast microscope. It empasizes slight differences in a specimen's capacity to bend light waves,enhancing light and darker regions without the use of stains.
The area of the specimen seen when looking through the microscope is the?
It depends on the particular microscope, and the magnification being used. Without these details, this question cannot be answered.
What characteristic of a microscope enales one to switch from one objective to another without altering the focus?
it is parfocal
Why does the stage of the microscope have an opening?
The Stage of a microscope has that little opening so that light can pass through the slide so you can see what's there. without it, you wouldn't be able to see the specimen on the slide.
Is there any difference between compound microscope and inverted microscope?
Special type of compound microscope, developed c. 1850, in which the specimen is illuminated from above and observed from below. A prism placed under the specimen reflects the light rays into body-tube. This feature made it possible to observe reactions without visual disturbance from the gases or the effervescence generated by them. so highly used in tissue culture experiments
What are the different types of lenses of an optical microscope?
The coarsest classification has the ocular or eyepieceand the objective.There are many designs for both these components of microscopes.The objective lens of a microscope is the one at the bottom near the sample. At its simplest it is a very high-powered magnifying glass, with very short focal length. This is brought very close to the specimen being examined so that the light from the specimen comes to a focus inside the microscope tube. The objective itself is usually a cylinder containing one or more lenses that are typically made of glass; its function is to collect light from the sample.An eyepiece consists of several "lens elements" in a housing, with a "barrel" on one end. The barrel is shaped to fit in a special opening of the instrument to which it is attached. The image can be focused by moving the eyepiece nearer and further from the objective. Most instruments have a focusing mechanism to allow movement of the shaft in which the eyepiece is mounted, without needing to manipulate the eyepiece directly.See the related links below.
How should a coverslip be applied to a drop of liquid on a microscope slide?
You have to put the cover slip on at an angle
What are oil immersion objective lens for?
because the oil have a refractive index near the glass one so we can work at high power without lossing any details by eliminating the air gap between the the specimen and the objective lens >>>> a.sh (iug)
