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Yes. Boiling water will kill lice and nits on a brush and comb. I would boil for 10 minutes but less time may be necessary to kill the lice and nits. You do not need to boil overnight. Lice and nits cannot survive in very high temperatures. Research has shown that lice and their eggs can survive freezing temperatures.

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Q: Can boiling water kill head lice on brush and comb?
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