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Q: Can dark field microscopy be used for living cells?
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What is Dark field microscopy?

Dark field microscopy (dark ground microscopy) describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. As a result, the field around the specimen (i.e. where there is no specimen to scatter the beam) is generally dark.


What is dark field?

Dark field microscopy (dark ground microscopy) describes microscopy methods, in both light and electron microscopy, which exclude the unscattered beam from the image. As a result, the field around the specimen (i.e. where there is no specimen to scatter the beam) is generally dark.


What type of microscopy is the negative stain similar to?

The darkfield microscope. "darkfield microscopy"


How is spirochete activity seen?

observation with dark-field microscopy .


What kind of technology does bright field dark field and phase microscopes use?

microscopy


What are Applications of bright field and dark field microscopy?

microscope is used to see small objects


What is darkfield microscopy?

A Dark Field microscope is one that has a special condensor (light source) that illuminates the specimen in such a way as to enhance the contrast even if it is unstained. When you look at a specimen in a dark field scope, it looks bright, but it is visible against a black background -- the 'dark field.'


Why use fluorescence microscopy for bacillus anthracis?

Perhaps fluorescence would be used because B. athracis has a cell wall making it difficult to visualize the details of the cytoplasm by simple bright field microscopy. Flourescence allows for labeling of specific entities, and "cold" illunimation of those entities against a dark field.


How is diagnosis for yaws conducted?

using a technique called dark-field microscopy. This often allows the spirochetes to be identified. They may also be identified in fluid withdrawn from swollen lymph nodes


Gram stain of lymes disease?

Lyme Disease is caused by Borrelia burgdorferi. This is a spirochete bacterium with a gram negative cell wall structure. These organisms are best viewed by dark-field microscopy.


What is a dark-field microscope used for?

Darkfield microscopes are useful in analyzing unstained tissue samples. Darkfield microscopy has also been used in computer mice, to help optical mice work on glass. It is also useful in the study of crystals and their defects.


A light microscope that makes the specimen appear light on a dark background is called a?

A darkfield microscope makes the specimen appear light on a dark background. It is an instrument used in light microscopy.