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Q: Can enterococci grow on CLED medium?
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How do you get rid of enterococci?

Some enterococci can be treated with penicillin, ampicillin, and vancomycin. Enterococci that are resistant to those drugs can be treated with Synercid.


Lab run out froom blood agar and cled plate what to do?

Depends on specimens you are inoculating - given CLED was mentioned I would assume urines. Standard set of PIM for a urine is HBA, MAC and CLED (although some labs omit the CLED). Most commonly encountered organisms in a UTI are enterobacteriaceae, especially E. coli; and other gram negative rods such as Pseudomonas. Can encounter Staphylococci and some other gram positive organisms - especially in hospital settings. Personally, in this situation I would use a CNA, Choc and MAC for urines - CNA grows the staphylococci (and streptococci and enterococci); Choc provides a non-selective media and MAC grows the gram negative rods that are most frequently isolated. Also, if nothing WAS isolated possibly release results with a comment about the accuracy of the results given the inavailbility of blood agar. Cled is useful for swarming proteus, to isolate them - I would attempt to isoalte and ID proteus without a CLED if necessary, if not put the plates aside (fridge) until a new supply of CLED can be brought in - if this will occur rapidly. If it will take longer consider shipping these specimens to another laboratory.


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