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What color is simple staining on a bacillus and coccus using methylene blue stain?
Methylene blue stains everything blue.
Is it possible to substitute methylene blue for nigrosin in the negative stain?
no
Which is an example of a dye used in a simple stain?
Methylene blue
Which substance can a student use to make the nuclei more visible?
A student can use a stain called methylene blue to make nuclei more visible under a microscope. Methylene blue is commonly used in biology and histology to stain cells and highlight structures like nuclei.
Is it useful to stain the Elodea and Anabaena cells with methylene blue stain?
Staining Elodea and Anabaena cells with methylene blue can be useful in microscopy to enhance contrast and visualize cell structures more clearly. Methylene blue is commonly used as a general stain to highlight cellular components such as nuclei and cytoplasm. This staining technique can aid in identifying cellular organelles and structures during microscopic examination.
How do you make methylene blue solution?
you can get methylene blue powder from a scientific store, it comes in powdered form. its pretty soluble in water and alcohol etc. the stain is made by dissolving an appropriate amount on methylene blue in a solvent, e.g for 0.1 dissolve 0.1% gram of methylene blue in 100 gram water, for 9% dissolve 9 grams
Can Methylene blue used to stain bacteria?
Yes because Methylene Blue is a symple stain which allows the staining of Cocci. The only thing that is done with the stain is to show the morphology of the bacteria, so one could tell the shape, size, and, arrangement.
What can you observe when you stain a cheek cell with methylene blue stain?
When staining a cheek cell with methylene blue, you can observe the cell's nucleus as a distinct blue color. The stain highlights the nucleus due to its affinity to bind with DNA. The rest of the cell may appear pale blue or colorless in comparison.
What is the counter or secondary stain used in the acid-fast stain technique?
The counter or secondary stain used in the acid-fast stain technique is methylene blue.
How long does a methylene blue skin stain last?
A methylene blue skin stain typically lasts for a few hours to a day, although it can vary based on the concentration used and individual skin characteristics. The stain can fade faster with rubbing or washing the area.
What is the composition of leishman stain?
Leishman stain is composed of a mixture of methylene blue and eosin Y dissolved in methanol. It is commonly used in laboratory settings to stain blood smears for microscopic examination, especially in the diagnosis of parasitic infections such as leishmaniasis.
Methylene blue can be prepared as a basic stain or an acidic stain. How would the pH affect the staining of bacteria?
When methylene blue is prepared as a basic stain, it will have a positive charge and selectively bind to negatively charged components of bacterial cells, such as nucleic acids, enhancing the staining of bacteria. On the other hand, if prepared as an acidic stain, it will have a negative charge and repel bacterial cells, resulting in poor staining of bacteria.
