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Formic acid for purines (A+G)Dimethyl sulfate for guanine and to some extent adenine
Hydrazine for Pyrimidines (C+T)
Addition of sodium chloride helps in separation of C and T
Hope this helps u :D
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∙ 11y ago
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Why are ddNTPs used for DNA sequencing?
Because on the addition of the normal ddNTP to the sequencing, there will be addition of another nucleotide added by DNA polymerase which keeps DNA sequencing going.
How many methods are used in DNA sequencing?
Since the birth of DNA sequencing in the 70's several methods have been developed which have become increasingly more efficient. There are probably 10-15 mainstream ways of sequencing, although dye-terminator sequencing is the one primarily used
What is gene sequencing used to determine?
When you hear gene sequencing you first think of DNA mapping. We think of paternity tests but gene sequencing has been used to determine diseases. More recently it has been used to directly help the patient with proper diagnoses.
What is the process used for DNA Sequencing?
There were initially two methods used for DNA sequencing, but today there are dozens. Genome sequencing is defined as any process that determines the order of nucleotides within an atom of DNA. It is almost always accomplished automatically in modern applications, using machines specifically designed for the job.
The sequencing rule that should be used to minimize average tardiness for a set of jobs to be processed on one machine is?
SPT
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
Why are ddNTPs used for DNA sequencing?
Because on the addition of the normal ddNTP to the sequencing, there will be addition of another nucleotide added by DNA polymerase which keeps DNA sequencing going.
How many methods are used in DNA sequencing?
Since the birth of DNA sequencing in the 70's several methods have been developed which have become increasingly more efficient. There are probably 10-15 mainstream ways of sequencing, although dye-terminator sequencing is the one primarily used
What is gene sequencing used to determine?
When you hear gene sequencing you first think of DNA mapping. We think of paternity tests but gene sequencing has been used to determine diseases. More recently it has been used to directly help the patient with proper diagnoses.
What is the 454 sequencing system?
Researching has shown that the 454 sequencing system is a scientific system that is used to process and identify different DNA's. This is a process that is used for analysis.
What does DNA isolation used for?
Gene sequencing and gene cloning
What types of jobs require sequencing worksheets?
Sequencing worksheets are used to put things an a particular order, usually chronological. They are often used by teachers as a tool to help with ordering skills.
Why would a person use Sanger sequencing?
Sangers sequencing technique is used to determine the order of nucleotides in a strand of DNA (deoxyribonucleic acid). It was first used to discover the order of the nucleotides in the genomes of various organisms during the Human Genome Project.
What is DNA sequencing used for?
determine the rick of developing certain diseases
What is the process used for DNA Sequencing?
There were initially two methods used for DNA sequencing, but today there are dozens. Genome sequencing is defined as any process that determines the order of nucleotides within an atom of DNA. It is almost always accomplished automatically in modern applications, using machines specifically designed for the job.
What is spatial sequencing?
EB: it's used in ordering sentences in a paragraph. :B
Is Gilbert a girls name or boys name?
Gilbert is most commonly used as a boys name.
