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Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
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How does DNA packaging affect gene expression?
controlling access to DNA
What is a method of making any copies of DNA?
Polymerase chain reaction (PCR) is the method of making copies of DNA.
The spools from which the DNA unwinds is made of what?
DNA is found on spools made of histones. These are groups of basic proteins found in the chromatin and involved in the packaging of DNA.
Dideoxyribonucleotide chain-termination is a method of?
Sequencing DNA (Sanger Sequencing Method).
What is the most commonly used method of cloning?
recombinant dna technology
What are the types of sequences?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
What are types of sequencing?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
How do you match a DNA sequence?
DNA sequence can be match by DNA sequencing method and it is of following type given by different scientist:1 The Sanger-Coulson method - this is chain termination method2 The Maxam-Gilbert method - this is chemical degratation of DNA3 By sequencing PCR products4 Automated DNA sequencing
What are the types of DNA?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
How did Walter Gilbert help with DNA?
Gilbert helped by developing a method for determining the sequence of nucleotides in the molecules of DNA and RNA.His work added a lot about how DNA, as a carrier of the genetic traits, directs the chemical machinery of the cell. He received the Nobel Prize in 1980.
What is DNA sequencing-maxam and Gilbert method?
DNA sequencing using the Maxam Gilbert method is now the less common of the two most known DNA sequencing methods. It is accurate, but very slow. It was first discovered some time in the mid 1900s. The Maxam Gilbert method relies on cutting billions of different DNA strands at various cites to produce different strands of discrete (each different) lengths. Imagine this: 5 - AATCGGTAGGCTACGTAGTCGACTGCATCGACTACG - 3. Now there are many As, Ts, Gs, Cs in this strand. However, the goal is to cut the strand starting at the 5' end into successive nucleotide sequences. You keep on doing this until you get these sequences: A AA AAT AATC AATCG - now remember so many cuts are going on (billions) that it actually works this out perfectly. These different DNA strands are then run through a DNA electrophoresis gel. There are millions of strands of equal lengths; millions of As AAs AATs and so on. These millions all migrate to the same place in the gel to create a very distinct band. Certain nucleotides (let's say on the G of AATCG) actually contain radioactive tags. This is how you tell the different nucleotides apart. This produces distinct bands on an x-ray film where the bands of DNA are (because the DNA contains a radioactive substance). You'll end up with a gel that looks like this: Take a ruler, go to each line and read what letter it is. Doing it this way, you'll get the genome (normally to around 500-800 base pairs). It takes a while, and that's why we all love the way computers do it all for us today.
How does DNA packaging affect gene expression?
controlling access to DNA
Is not a good analogy for the packaging problem encountered by DNA?
File compression
What Is a good analogy for the packaging problem encountered by DNA?
vacuum deflation
How is DNA tested and why?
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
What is a method of making any copies of DNA?
Polymerase chain reaction (PCR) is the method of making copies of DNA.
Where do you get the DNA fragments for Gel electrophoresis?
The DNA fragments comes from the method of DNA isolation.
