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Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
What else can I help you with?
How does DNA packaging affect gene expression?
DNA packaging plays a crucial role in gene expression as the compacted structure of chromatin can restrict the accessibility of transcription factors and RNA polymerase to DNA, thus reducing gene expression. Conversely, when DNA is more loosely packaged, such as in an open chromatin state, it allows for easier access to the transcriptional machinery and enhances gene expression. Therefore, the level of DNA packaging can directly influence the regulation of gene expression.
Which method is preparing DNA for forensic analysis?
The method commonly used for preparing DNA for forensic analysis is called polymerase chain reaction (PCR). PCR amplifies specific regions of DNA so that they can be analyzed in detail. This method allows for small amounts of DNA to be replicated, making it suitable for forensic samples with limited DNA material.
What is a method of making any copies of DNA?
One method of making copies of DNA is through a process called polymerase chain reaction (PCR). In PCR, a DNA template is mixed with primers, nucleotides, and DNA polymerase, and subjected to cycles of heating and cooling to amplify the target DNA region. This results in millions of copies of the DNA target.
Which is a method to prepare DNA for forensic analysis?
One method to prepare DNA for forensic analysis is called DNA extraction. This involves isolating DNA from the sample using various techniques, such as chemical or mechanical disruption of cells, enzymatic digestion, and purification steps to obtain high-quality DNA for analysis.
Dideoxyribonucleotide chain-termination is a method of?
Dideoxyribonucleotide chain-termination is a method used in DNA sequencing to determine the sequence of nucleotides in a DNA molecule. It involves terminating DNA synthesis at specific bases by incorporating dideoxyribonucleotides (ddNTPs) into the growing DNA strand, which lack the 3' hydroxyl group needed for further elongation. This results in a series of fragments of varying lengths that can be separated by size to reveal the DNA sequence.
What are types of sequencing?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
What are the types of sequences?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
How do you match a DNA sequence?
DNA sequence can be match by DNA sequencing method and it is of following type given by different scientist:1 The Sanger-Coulson method - this is chain termination method2 The Maxam-Gilbert method - this is chemical degratation of DNA3 By sequencing PCR products4 Automated DNA sequencing
How did Walter Gilbert help with DNA?
Gilbert helped by developing a method for determining the sequence of nucleotides in the molecules of DNA and RNA.His work added a lot about how DNA, as a carrier of the genetic traits, directs the chemical machinery of the cell. He received the Nobel Prize in 1980.
What are the types of DNA?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
What is DNA sequencing-maxam and Gilbert method?
DNA sequencing using the Maxam Gilbert method is now the less common of the two most known DNA sequencing methods. It is accurate, but very slow. It was first discovered some time in the mid 1900s. The Maxam Gilbert method relies on cutting billions of different DNA strands at various cites to produce different strands of discrete (each different) lengths. Imagine this: 5 - AATCGGTAGGCTACGTAGTCGACTGCATCGACTACG - 3. Now there are many As, Ts, Gs, Cs in this strand. However, the goal is to cut the strand starting at the 5' end into successive nucleotide sequences. You keep on doing this until you get these sequences: A AA AAT AATC AATCG - now remember so many cuts are going on (billions) that it actually works this out perfectly. These different DNA strands are then run through a DNA electrophoresis gel. There are millions of strands of equal lengths; millions of As AAs AATs and so on. These millions all migrate to the same place in the gel to create a very distinct band. Certain nucleotides (let's say on the G of AATCG) actually contain radioactive tags. This is how you tell the different nucleotides apart. This produces distinct bands on an x-ray film where the bands of DNA are (because the DNA contains a radioactive substance). You'll end up with a gel that looks like this: Take a ruler, go to each line and read what letter it is. Doing it this way, you'll get the genome (normally to around 500-800 base pairs). It takes a while, and that's why we all love the way computers do it all for us today.
Chemicals used in maxam Gilbert sequencing?
Formic acid for purines (A+G)Dimethyl sulfate for guanine and to some extent adenine Hydrazine for Pyrimidines (C+T) Addition of sodium chloride helps in separation of C and T Hope this helps u :D
DNA wrapping around histones is an example of what type of packaging process?
DNA wrapping around histones is an example of chromatin packaging. Histones are proteins around which DNA is wound to form nucleosomes, which enable compaction of DNA into a smaller space. This packaging process helps regulate gene expression and DNA accessibility.
How is DNA tested and why?
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
What Is a good analogy for the packaging problem encountered by DNA?
vacuum deflation
Is not a good analogy for the packaging problem encountered by DNA?
File compression
How does DNA packaging affect gene expression?
DNA packaging plays a crucial role in gene expression as the compacted structure of chromatin can restrict the accessibility of transcription factors and RNA polymerase to DNA, thus reducing gene expression. Conversely, when DNA is more loosely packaged, such as in an open chromatin state, it allows for easier access to the transcriptional machinery and enhances gene expression. Therefore, the level of DNA packaging can directly influence the regulation of gene expression.
