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DNA sequencing using the Maxam Gilbert method is now the less common of the two most known DNA sequencing methods. It is accurate, but very slow. It was first discovered some time in the mid 1900s.
The Maxam Gilbert method relies on cutting billions of different DNA strands at various cites to produce different strands of discrete (each different) lengths. Imagine this: 5 - AATCGGTAGGCTACGTAGTCGACTGCATCGACTACG - 3.
Now there are many As, Ts, Gs, Cs in this strand. However, the goal is to cut the strand starting at the 5' end into successive nucleotide sequences.
You keep on doing this until you get these sequences:
A
AA
AAT
AATC
AATCG - now remember so many cuts are going on (billions) that it actually works this out perfectly.
These different DNA strands are then run through a DNA electrophoresis gel. There are millions of strands of equal lengths; millions of As AAs AATs and so on. These millions all migrate to the same place in the gel to create a very distinct band.
Certain nucleotides (let's say on the G of AATCG) actually contain radioactive tags. This is how you tell the different nucleotides apart. This produces distinct bands on an x-ray film where the bands of DNA are (because the DNA contains a radioactive substance).
You'll end up with a gel that looks like this: Take a ruler, go to each line and read what letter it is. Doing it this way, you'll get the genome (normally to around 500-800 base pairs). It takes a while, and that's why we all love the way computers do it all for us today.
What else can I help you with?
How did Walter Gilbert help with DNA?
Gilbert helped by developing a method for determining the sequence of nucleotides in the molecules of DNA and RNA.His work added a lot about how DNA, as a carrier of the genetic traits, directs the chemical machinery of the cell. He received the Nobel Prize in 1980.
What are types of sequencing?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
What are the types of sequences?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
What is maxam Gilbert method of DNA packaging?
Used in DNA sequencing; four samples of end-labeled DNA restriction fragments are chemically cleaved at different specific nucleotides. The resulting subfragments are separated by gel electrophoresis, and the labeled fragments are detected by autoradiography. The sequence of the original end-labeled restriction fragment can be determined directly from parallel electropherograms of the four samples
How do you match a DNA sequence?
DNA sequence can be match by DNA sequencing method and it is of following type given by different scientist:1 The Sanger-Coulson method - this is chain termination method2 The Maxam-Gilbert method - this is chemical degratation of DNA3 By sequencing PCR products4 Automated DNA sequencing
How is DNA tested and why?
by DNA fingerprinting method , DNA-DNA hybirdization or DNA sequencing. to know the sequence of DNA
What are the types of DNA?
THERE ARE TWO TYPES OF DNA SEQUENCING......1) MAXAM-GILBERT METHOD (OR) CHEMICAL METHOD...2) SANGER DI-DEOXY METHOD...3) Shotgun sequencing4) Primer walking
Which method is preparing DNA for forensic analysis?
The method commonly used for preparing DNA for forensic analysis is called polymerase chain reaction (PCR). PCR amplifies specific regions of DNA so that they can be analyzed in detail. This method allows for small amounts of DNA to be replicated, making it suitable for forensic samples with limited DNA material.
What is a method of making any copies of DNA?
One method of making copies of DNA is through a process called polymerase chain reaction (PCR). In PCR, a DNA template is mixed with primers, nucleotides, and DNA polymerase, and subjected to cycles of heating and cooling to amplify the target DNA region. This results in millions of copies of the DNA target.
What method is used to make DNA fingerprints?
A method known as RFLP (restriction fragment length polymorphism) analysis can be used to make a DNA fingerprint.
NOT a likely method of DNA replication as determined by the structure of DNA?
Strand displacement replication is not a likely method of DNA replication because it involves the formation of multiple replication forks which is not supported by the structure of DNA, which consists of two antiparallel strands.
What has the author Neal Ward Gilbert written?
Neal Ward Gilbert has written: 'Renaissance concepts of method' -- subject(s): Methodology, Philosophy, Renaissance, Renaissance Philosophy
