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Crystal violet acid-base indicator

Updated: 5/23/2024
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Crystal violet is a triphenylmethane dye that can act as an acid-base indicator. In acidic solutions, it appears as a red color, while in basic solutions, it turns blue or violet. This color change is due to the different protonation states of the dye molecule at different pH levels.

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What is the secondary stain in gram stain procedure?

It is crystal violet & stains all cells purple.


What will be the color change of crystal violet at different pH?

Crystal violet is a pH indicator that changes color depending on the pH of the solution. At lower pH values (acidic conditions), crystal violet appears yellow, while at higher pH values (alkaline conditions), it changes to a blue color. This color change makes it useful for determining the pH of a solution within a specific range.


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Traditional antifungal drugs such as.gentian violet (also known as crystal violet)


What is the name of the acide base color indicator found in MacConkey agar?

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Does crystal violet have a color?

Yes, crystal violet is a triarylmethane dye that appears as a deep purple color when dissolved in water or other solvents.


What is crystal violet iodine complex?

In Gram staining procedure during bacterial staining , iodine forms a complex with crystal violet stain which stains Gram positive bacteria blue to violet .


Why is Iodine used in Gram staining?

Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.


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Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.


Is crystal violet used as carcinogenic agent?

Crystal violet is not classified as a known carcinogen, but it has been shown to have genotoxic and mutagenic properties in some studies. It is important to handle crystal violet with caution and follow proper safety protocols when working with this compound in the lab.


What color would the bacterial cell have when viewed microscopically after the treatment with ammonium oxalate crystal violet?

The bacterial cell would appear purple when viewed microscopically after treatment with ammonium oxalate crystal violet. The crystal violet binds to the peptidoglycan layer of the cell wall, causing it to retain the purple color even after subsequent washing steps.


Why do you add crystal violet stain in simple staining method?

Because violet dye is the least expensive.