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No, what a stupid thing to say. Crystals have nothing to do with carcinogenic agents and when you use the 'Violet' are you trying to subtlely insult my mother, who is called Violet
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∙ 14y ago
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What are the two techniques used to visualize endospores?
The crystal violet method and the Schaeffer-Fulton method
What are the two common techniques used to visualize endospores?
The crystal violet method and the Schaeffer-Fulton method
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
Is Titanium Dioxide healthy for you?
No. But it's not particularly harmful either. It's a coloring agent, used to color food and cosmetic products white.
Function of crystal violet?
Crystal violet is a hexamethyl also known as methyl violet 10B This is much darker than 2B, and often darker than 6B. It is used in biological stainang particularly gram staining together with safranin and iodine. Crystal violet was also used to treat strept throat. Doctors used to swab it on the back of your throat.
Why is Iodine used in Gram staining?
Iodine is added as a mordant to enhance crystal violet staining by forming a crystal violet-iodine complex.
What will be the color change of crystal violet at different pH?
Crystal violet is used in Gram's method of bacteria classification. At a pH of 1.0, the solution turns green, and when in a strongly acidic solution, it turns yellow.
What are the two techniques used to visualize endospores?
The crystal violet method and the Schaeffer-Fulton method
What are the two common techniques used to visualize endospores?
The crystal violet method and the Schaeffer-Fulton method
What is the action of iodine in grams stain?
Iodine is used to bind the Crystal Violet to the Gram Positive microbes.
Is crystal violet a basic or an acidic dye?
Crystal violet, also called methyl violet 10B, is a dark purple staining dye used in biology and microbiology which, like many staining dyes, is acidic.The acidic nature of crystal violet dye has many implications in chemistry, determining how it will interact with other substances, which microorganisms will absorb or repel it, and how it will effect structures such as cell walls or DNA.Since many acidic staining dyes are caustic or carcinogenic, they can pose logistical, legal, and ethical problems during disposal. Numerous studies have been launched in order to find cost-effective ways to degrade, absorb, neutralize, or deactivate these chemicals.Knowlege of the acidic nature of such substances is among several important considerations in devising such approaches.
What is the chemical nature of crystal violet dye?
Crystal violet is the 'methyl violet 10B' derivative (with six methyl groups) of a difficult to draw structural chemical formula. (see related link, C26H34N3Cl).It is known in medicine as Gentian violet (or crystal violet) and is the active ingredient in a Gram stain, used to identify bacteria. It is toxicIt inhibits growth of Gram positives except streptococci.Methyl violet also binds to DNA. This means it can be used in cell viability assays in biochemistry. However, this binding to DNA will cause replication errors in living tissue, possibly leading to mutations and cancer.
Function of acetone in gram staining?
Acetone is used for decolouring method, washing away the iodine-crystal violet complex formed in gram negative bacteria. so that the gram negative bacteria can be then stained with safranin or fuchsin.
What is the dogtooth violet bulb used for?
In Japan, dogtooth violet bulbs used to be made into katakuriko, a thickening agent for cooking. Nowadays, potato starch or cornstarch are often used as a substitute for katakuriko, since natural dogtooth violet starch has become very expensive.
Function of Gram's iodine?
Iodine is used as a mordant in the gram staining procedure to make large crystals when it is used with crystal violet dye. In gram positive cell walls those crystals get stuck and wont get washed off with the alcohol. In gram negative cell walls the crystals are washed out.
What happens if grams iodine is not applied after staining with crystal violet?
If the iodine in the Gram's stain was omitted, the crystal violet which enters the cell will not be able to form the Crystal Violet Iodine (CVI) complex. The CVI complex molecule has a larger size than that of Crystal violet molecule and hence Gram +ve bacteria are able to retain the stain. If iodine were omitted, even Gram+ve cells would get decolorised by Alcohol treatment and would be seen as Gram-ve cells.
