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Crystal violet, also called methyl violet 10B, is a dark purple staining dye used in Biology and microbiology which, like many staining dyes, is acidic.
The acidic nature of crystal violet dye has many implications in chemistry, determining how it will interact with other substances, which microorganisms will absorb or repel it, and how it will effect structures such as cell walls or DNA.
Since many acidic staining dyes are caustic or carcinogenic, they can pose logistical, legal, and ethical problems during disposal. Numerous studies have been launched in order to find cost-effective ways to degrade, absorb, neutralize, or deactivate these chemicals.
Knowlege of the acidic nature of such substances is among several important considerations in devising such approaches.
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Hematoxylin is an acidic dye or basic dye?
Hematoxylin is an basic dye!
Is saponin an acidic or basic dye?
saponin is not a dye rather it is a surfactant produced by plant.
Do basic solutions turn the dye litmus red?
Red color in acidic solutions and blue color in basic solutions.
Which ion carries the chromophore in an acidic dye?
The negative ion
Litmus solution is purple dye which is is extracted from?
Litmus solution is obtained from lichens. It is used to test whether the solution is acidic or basic.
Hematoxylin is an acidic dye or basic dye?
Hematoxylin is an basic dye!
Is carbol fuschin an acidic dye?
No. It is basic. No. It is basic.
What bacterial staining technique is it when a basic dye is utilized to stain bacterial cells?
A basic dye used in gram staining is crystal violet.
Why do you add crystal violet stain in simple staining method?
Because violet dye is the least expensive.
Why add saffranin?
Saffranin is a " counter stain " . this is also a " basic dye " which is in different color than primary stain ( basic dye crystal violet ) . The purpose counter stain ( saffranin ) is to give the decolourised cells a color , that is different from first one ( crystal violet ). The microbes which are not decolourised by alcohol retains the stain, while the decolourised cell take up the counter stain ( saffranin ).
What is the principle of crystal violet staining in monolayer culture?
Crystal Violet is a dye that accumulates in the cell nucleus. The fixed dye, measured photometrically after solubilization, correlates with the nuclear DNA content and thus with cell number.
Is saponin an acidic or basic dye?
saponin is not a dye rather it is a surfactant produced by plant.
Is safranin an acidic dye?
Sure, any basic stain can be used for simple, direct staining.
What the principle of leishman stain?
It is basically use to stain leukocytes,maleria prasite and trypanosomas. leisman stain contain 1st methylene blue dye, a basic dye, which gives color to an acidic component.2nd eosin dye,an acidic dye ,which gives color to a basic component. These dye differentiat the different component of blood.
What is the principle of leishman stain?
It is basically use to stain leukocytes,maleria prasite and trypanosomas. leisman stain contain 1st methylene blue dye, a basic dye, which gives color to an acidic component.2nd eosin dye,an acidic dye ,which gives color to a basic component. These dye differentiat the different component of blood.
Crystal violet in the MacConkey agar?
MacConkey's agar refers to a culture medium that is designed to selectively grow Gram-negative bacteria. It contains crystal violet dye, which inhibits certain Gram-positive bacteria.
Is anthraquinone violet dye and methyl violet dye the same?
yes
