DNA extraction from bacteria?
DNA extraction from bacteria can be achieved in various ways. Yeast is the best resource to extract the DNA bacteria from using extreme rapid extraction method.
During DNA extraction the following precautions have to be taken - 1. All steps of the extraction must be conducted in a sterile condition in a laboratory to avoid contamination. 2. DNA extract is used for DNA extraction. DNA extracts contains irritants, handle with care. Wear goggles to protect your eyes against any splashes. 3. Alcohol is used during DNA extraction. Alcohol catches fire very easily, keep alcohol away from heat.
DNA extraction is done by three methods: * Organic extraction * inorganic extraction * solid state method In organic extraction, phenol and chloroform are used to create on organic phase in which cells are lysed and DNA is freed. The DNA remains in the aqueous phase. Ethyl alcohol is used to precipitate the DNA. In the inroganic methos, NaCl and EDTA are used for cell lysis. Following this, an approach similar to the organic method…
The glyco-callyx (sugar-protein) covering of the eukaryotic Cell Coat has much less strength than the mostly cellulose (comprised of strongly bound - bacterially released - glucose) based Cell Wall of the prokaryotic bacteria. Dna extraction is more difficult in prokaryotes due to the tough Cell Wall.