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Cell culture is the complex process by which cells are grown under controlled conditions. The growth of explants taken directly form the living organism (e.g. biopsy material) is also known as primary cell culture. The culture consists of mixed population of cell types. Frequently the some of the cells may survive without proliferating and will therefore be lost in the increasing population of those which are able to multiply in the conditions supplied in vitro. Cells from explants may sometimes be converted to cell lines by passage. These may continue to proliferate for a number of cell generations. In some instances the primary cells are fused with so-called immortal (cancer) cells to produce a hybridoma line. Many of the explanted cells will only survive for one or a few passages before dying.

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12y ago
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9y ago

Cells and batteries are categorised as being 'primary' or 'secondary'. A 'primary cell' is non-rechargeable -in other words, it is a disposable cell. A 'secondary cell' is a rechargeable cell.

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14y ago

The difference is that the primary cell wall is more flexible, while the secondary cell wall is sturdier.

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Q: Define the difference between a primary and secondary cell?
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